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Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-10 16:01:13.530
Product last modified at: 2024-11-21T22:00:10.016Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Myddosome Complex Antibody Sampler Kit

Myddosome Complex Antibody Sampler Kit #39433

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Myddosome Complex Antibody Sampler Kit provides an economical means of detecting the components of the myddosome complex using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Myddosome Complex Antibody Sampler Kit detects endogenous levels of its target human protein. Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb recognizes endogenous levels of IRAK4 protein when phosphorylated at Thr345 and Ser346. This antibody shows slight reactivity with IRAK4 when singly phosphorylated at Ser346 and does not cross-react with IRAK4 singly phosphorylated at Thr345. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated at Ser172 and may cross-react with phospho-IKKε.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to a central region of mouse TRAF6 proteins, with synthetic phosphopeptides corresponding to residues surrounding Thr345/Ser346 and Ser172 of human IRAK4 and TBK1/NAK1 proteins, respectively, and with synthetic peptides corresponding to residues surrounding Cys233 of human MyD88 protein and residues near the carboxyl termini of mouse IRAK1 and human TBK1/NAK protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues at the carboxy terminus of mouse IRAK2 and residues surrounding Lys41 of human IRAK4. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Toll-like receptors (TLRs) are a large family of so-called pattern recognition receptors (PRRs) that detect pathogen associated molecular patterns (PAMPs) and danger associated molecular patterns (DAMPs) (1,2). Upon activation, TLRs initiate two main signaling pathways through their C-terminal cytoplasmic Toll/Il-1 receptor (TIR) domain that couples with TIR domain-containing adaptors MyD88 and TRIF. The MyD88-dependent pathway is initiated by the formation of a large oligomeric protein complex termed myddosome. Myddosome is one of so-called supramolecular organizing centers (SMOCs), a signaling organelle that is common for PRRs in the innate immune system. Myddosome formation promotes IRAK4 activation, which in turn activates IRAK1 and later, IRAK2. TRAF6 is then recruited and activated through the binding sites within IRAKs. Activated TRAF6 is released to the cytosol and triggers the IKK complex to activate the NF-κB pathway to mediate the expression of pro-inflammatory cytokines and chemokines (3-7). Recently, it was also found that TBK1 is recruited to the myddosome complex and activated to induce aerobic glycolysis (8).

    Database Links

    UniProt ID: Q99836 , O43187 , P51617 , Q9NWZ3 , Q9UHD2 , P70196


    Entrez-Gene Id: 4615 , 3656 , 3654 , 51135 , 29110 , 22034


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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