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Render Timestamp: 2025-01-22T10:19:47.266Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:58:33.831
Product last modified at: 2025-01-01T09:06:46.986Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SARS-CoV-2 Spike Protein (S2) (E7V3M) Rabbit mAb #27620

Filter:
  • WB
Western Blotting Image 1: SARS-CoV-2 Spike Protein (S2) (E7V3M) Rabbit mAb
Western blot analysis of extracts from Vero-E6 cells, mock-infected (lane 1) or infected with SARS-CoV-2 (lane 2), using SARS-CoV-2 Spike Protein (S2) (E7V3M) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The antibody detects full-length (uncleaved) SARS-CoV-2 spike protein, and the fragment corresponding to the S2 domain generated by endogenous protease cleavage. SARS-CoV-2 infected Vero-E6 cells courtesy of Dr. Mohsan Saeed, National Emerging Infectious Diseases Laboratories, Boston University.

To Purchase # 27620

Cat. #

Size

27620T
20 µl
27620S
100 µl

Supporting Data

REACTIVITY Vir
SENSITIVITY Endogenous
MW (kDa) 100, 220
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Vir-Virus 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SARS-CoV-2 Spike Protein (S2) (E7V3M) Rabbit mAb recognizes endogenous levels of total SARS-CoV-2 spike protein. This antibody detects full-length (uncleaved) SARS-CoV-2 spike protein and the fragment corresponding to the S2 domain generated by host protease cleavage. It also detects full-length (uncleaved) SARS-CoV-1 spike protein, but does not cross-react with spike proteins from MERS coronavirus.

Species Reactivity:

Virus

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1140 of SARS-CoV-2 spike protein.

Background

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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