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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Stress Granule Marker IF Antibody Sampler Kit

Stress Granule Marker IF Antibody Sampler Kit #29530

Stress Granule Marker IF Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using G3BP2 Antibody.
1/51

To Purchase # 29530

Cat. # Size Qty. Price
29530T 1 Kit
5 x 20 microliters
$489
  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
G3BP1 (E9G1M) XP® Rabbit mAb #6155920 µlWB, IP, IHC, IFH Mk68Rabbit IgG
G3BP2 Antibody #3179920 µlWB, IP, IFH M R Mk60, 70Rabbit 
TIAR (D32D3) XP® Rabbit mAb #850920 µlWB, IP, IF, F, eCLIPH M R Mk42Rabbit IgG
FMRP (D14F4) Rabbit mAb #710420 µlWB, IFH M R Mk80Rabbit IgG
UBAP2L (E5X4E) Rabbit mAb #4019920 µlWB, IP, IHC, IF, eCLIPH M Mk150, 160Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 

Product Information

Kit Usage Information

Protocols

Product Description

The Stress Granule Marker IF Antibody Sampler Kit provides an economical means of evaluating total levels of stress granule-associated proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Background

Stress granules (SGs) are cytoplasmic condensates that form at sites of stalled mRNA translation in response to various cellular stressors. SGs are composed of translationally inactive 48S preinitiation complexes (PICs), untranslated mRNA, and a complex mixture of RNA-binding proteins (RBPs). Two key mediators of SG assembly are the RBPs Ras-GTPase-activating protein-binding proteins 1 and 2 (G3BP1/2), which bind to excess mRNA and recruit additional SG-associated proteins. Overexpression of either G3BP1 or G3BP2 is enough to induce SG formation even in the absence of stress, and reduced levels of G3BP1 or G3BP2 protein severely inhibit SG formation, highlighting their crucial role in this process (1-4). Additional SG-associated proteins include TIA-1-related protein (TIAR), fragile X mental retardation protein (FMRP), and ubiquitin-associated protein 2-like (UBAP2L). TIAR is a member of the RNA-recognition motif (RRM) family of RBPs (5,6). In response to cellular stress, TIAR associates with G3BP1/2 and its family member TIA-1 to form SG condensates (7,8). The two major isoforms of TIAR are the products of alternative mRNA splicing (9,10). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (11-13). Each of these related proteins can associate with one another as well as form homodimers (13). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (14). In addition, FMRP, FXR1, and FXR2 are components of SGs and have been implicated in the translational regulation of mRNAs (15). UBAP2L is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (16). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (17). UBAP2L is essential for the formation of SGs, and some studies suggest that arginine methylation by PRMT1 inhibits UBAP2L interaction with SG elements and overall SG assembly (18-20).
  1. Tourrière, H. et al. (2003) J Cell Biol 160, 823-31.
  2. Matsuki, H. et al. (2013) Genes Cells 18, 135-46.
  3. Kedersha, N. et al. (2016) J Cell Biol 212, 845-60.
  4. Yang, P. et al. (2020) Cell 181, 325-345.e28.
  5. Tian, Q. et al. (1991) Cell 67, 629-39.
  6. Kawakami, A. et al. (1992) Proc Natl Acad Sci USA 89, 8681-5.
  7. Anderson, P. and Kedersha, N. (2002) Cell Stress Chaperones 7, 213-21.
  8. Anderson, P. and Kedersha, N. (2002) J Cell Sci 115, 3227-34.
  9. Kawakami, A. et al. (1994) J Immunol 152, 4937-45.
  10. Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.
  11. Verkerk, A.J. et al. (1991) Cell 65, 905-14.
  12. Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
  13. Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
  14. Caudy, A.A. et al. (2002) Genes Dev 16, 2491-6.
  15. Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.
  16. Wilde, I.B. et al. (2011) J Proteome Res 10, 1062-72.
  17. Bordeleau, M.E. et al. (2014) Blood 124, 2362-9.
  18. Markmiller, S. et al. (2018) Cell 172, 590-604.e13.
  19. Cirillo, L. et al. (2020) Curr Biol 30, 698-707.e6.
  20. Huang, C. et al. (2020) Cell Death Differ 27, 227-241.

Database Links

UniProt ID: Q01085 , Q06787 , Q13283 , Q14157 , Q9UN86


Entrez-Gene Id: 7073 , 2332 , 10146 , 9898 , 9908


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