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XML generation date: 2024-09-20 06:16:59.088
Product last modified at: 2024-09-13T07:01:05.328Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. TRAF6 Antibody

TRAF6 Antibody #4743

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Product Name

8028 TRAF6 (D21G3) Rabbit mAb
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  • WB
  • IP

Inquiry Info. # 4743

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRAF6 Antibody detects endogenous levels of total TRAF6 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a sythetic peptide corresponding to resides near the amino terminus of human TRAF6. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
    TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (5). TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development (7). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (8) that results in signaling responses from the interleukin-1 receptor (IL-1R) (9), toll-like receptor (10,11), CD40 (12), RANK (13,14), and p75 neurotrophin receptor (15). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (10). It leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex (16). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (17).
    1. Arch, R.H. et al. (1998) Genes Dev 12, 2821-30.
    2. Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88.
    3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
    4. Rothe, M. et al. (1994) Cell 78, 681-92.
    5. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-1105.
    6. Lomaga, M.A. et al. (1999) Genes Dev. 13, 1015-1024.
    7. Lomaga, M.A. et al. (2000) J. Neurosci. 20, 7384-7393.
    8. Ye, H. et al. (2002) Nature 418, 443-447.
    9. Cao, Z. et al. (1996) Nature 383, 443-446.
    10. Muzio, M. et al. (1997) Science 278, 1612-1615.
    11. Medzhitov, R. et al. (1998) Mol .Cell 2, 253-258.
    12. Ishida, T. et al. (1996) J. Biol. Chem. 271, 28745-28748.
    13. Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-20555.
    14. Wong, B.R. et al. (1998) J. Biol. Chem. 273, 28355-28359.
    15. Khursigara, G. et al. (1999) J. Biol. Chem. 274, 2597-2600.
    16. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
    17. Wong, B.R. et al. (1999) Mol. Cell 4, 1041-1049.

    Database Links

    UniProt ID: Q9Y4K3


    Entrez-Gene Id: 7189


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