In spatial biology, multiplexing in immunohistochemistry (IHC) enables the simultaneous detection of multiple biomarkers within a single tissue section. This unravels insights into how cellular interactions influence the tissue microenvironment, deepening our understanding of disease progression and therapeutic responses.
SignalStar™ Multiplex IHC is a technology that uses antibodies, oligonucleotides, and fluorophores to interrogate cellular presence, location, function, and biomarker co-expression patterns. Multiple biomarkers can be amplified simultaneously in FFPE tissue with high sensitivity and specificity, providing results for up to eight biomarkers in two days.
SignalStar mIHC panels combine CST scientific expertise, our high-quality antibodies, and rigorous validation into an easy-to-use solution that enables you to generate multiplex spatial images and deepen your understanding of biology, even when you’re not a spatial expert. So you can easily design experiments and get answers to your most pressing scientific questions—fast.
You can design a SignalStar mIHC panel specific to your needs, or reference human-reactive and mouse-reactive multiplex IHC panel designs curated by CST scientists.
Click on the panels below to learn more.
Detect immune cell types in a human tumor microenvironment (TME).
Biomarker | Relevance |
---|---|
Pan-Keratin | Epithelium (identifies tumor cells) |
CD3 | All T cells |
CD4 | Helper T cells |
CD8 | Cytotoxic T cells |
FoxP3 | Regulatory T cells |
CD68 | Macrophages |
CD11c | Dendritic cells |
CD20 | B cells |
Gain a comprehensive understanding of T cell dynamics within the TME.
Biomarker | Relevance |
---|---|
Granzyme B | Cytotoxic potential of T cells |
CD3 | All T cells |
CD8 | Cytotoxic T cells |
CD4 | Helper T cells |
PD-1 | Stem-like T cells, exhausted T cells |
TIM3 | Exhausted T cells |
LAG3 | Exhausted T cells |
TCF1 | Stem-like T cells |
Observe myeloid cells and relevant functional markers.
Biomarker | Relevance |
---|---|
CD11b | Myeloid cells |
CD11c | M1-polarized macrophage |
CD68 | Macrophages |
CD206 | M2-polarized macrophage |
CD163 | M2-polarized macrophage |
HLA-DRA | M1-polarized macrophage |
XCR1 | Conventional DC1 (CD8 T cell antigen) |
CD86 | M1-polarized macrophage |
Gain detailed insights into immune cell infiltration versus immune exclusion within the TME.
Biomarker | Relevance |
---|---|
Pan-Keratin | Epithelium (identifies tumor cells) |
CD3 | All T cells |
CD8 | Cytotoxic T cells |
FoxP3 | Regulatory T cells |
F4/80 | Macrophages |
CD11b | Myeloid cells |
Ly6G | Neutrophils and MDSCs (myeloid-derived suppressor cells) |
CD19 | B cells |
Observe myeloid cells and relevant functional markers.
Biomarker | Relevance |
---|---|
CD11b | Myeloid cells |
F4/80 | Macrophages |
CD206 | M2-polarized macrophage |
CD86 | M1-polarized macrophage |
Arginase | M2-polarized macrophage |
Ly6G | Neutrophils and polymorphonuclear MDSCs |
SIRPɑ | Macrophage inhibitory receptor |
Technical Support
Visit the Technical Support page to search for troubleshooting information and answers to technical questions.
Cell Signaling Technology, CST, and SignalStar are trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.