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3/28/2025, 10:24:44 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
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Control Cell Extracts

These positive and negative control cell extracts and proteins are made and used in-house for antibody validation and lot to lot testing. We recommend using these extracts as control lanes on your western blot, to ensure that western blotting procedures are appropriately executed. The use of proper controls also facilitates troubleshooting, as problems can then be attributed to either the western blotting protocol or the quality of the extracts used in the actual experiment.

For more information, please also see our controls table, which compiles cell lines and treatments that can be used as a positive control for our activation-state specific antibodies and serve as a reference for appropriately controlled experiments.

Control Cell Extracts

No.

Name

9273Akt Control Cell Extracts
9158AMPK Control Cell Extracts
9223ATF-2 Control Cell Extracts
9263c-Jun Control Cell Extracts
9663Caspase-3 Control Cell Extracts
9193CREB Control Cell Extracts
5634EGF Receptor Control Cell Extracts
2043Jurkat Apoptosis Cell Lysates (etoposide)
11972LC3 Control Cell Extracts
9160MEK1/2 HeLa Control Cell Extracts
9233MKK3/MKK6 Control Cell Extracts
9243NF-κB Control Cell Extracts
9213p38 MAPK Control Cell Extracts
9194p44/42 MAPK (Erk1/2) Control Cell Extracts
9203p70 S6 Kinase Control Cell Extracts
7988PathScan® Sandwich ELISA Control Phospho Cell Extract I
7989PathScan® Sandwich ELISA Control Phospho Cell Extract II
9253SAPK/JNK Control Cell Extracts
12052Smad2/3 Control Cell Extracts
9173Stat1 Control Cell Extracts
9133Stat3 Control Cell Extracts
9353Stat5 Control Cell Extracts

Control Proteins

No.

Name

9293Bad Control Proteins
9113cdc2 (Tyr15) Control Proteins
9183Elk-1 Control Proteins
9103p44/42 MAPK (Erk1/2) Control Proteins
9303Rb Control Proteins
2904VEGF Receptor 2 Control Proteins