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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TFE3 (E6T9M) Rabbit mAb #81744

Filter:
  • WB
  • IP
Western blot analysis of extracts from control U-2 OS cells (lane 1) or CRISPR/Cas9 TFE3 isoform 1 knockout (KO) cells (lane 2) using TFE3 (E6T9M) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 81744

Cat. # Size Qty. Price
81744T 20 µl
$168
81744S 100 µl
$371

Product Specifications

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 85-89
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TFE3 (E6T9M) Rabbit mAb recognizes endogenous levels of total TFE3 protein. This antibody does not detect isoform 2 of TFE3.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu59 of human TFE3 protein.

Background

Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that include MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the TFE3 gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the TFE3 gene have been described and characterized in detail (5).
For Research Use Only. Not for Use in Diagnostic Procedures.
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