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Human Anti-CD3/CD28 T Cell Activation Kit #70976

Human Anti-CD3/CD28 T Cell Activation Kit: Image 1
Western blot analysis of extracts from Jurkat cells, untreated (lane 1) or treated with Human Anti-CD3/CD28 T Cell Activation Kit #70976 (30 min) (lane 2), using Phospho-SLP-76 (Ser376) (D7S1K) Rabbit mAb #92711 (upper), SLP-76 (E4N7E) Rabbit mAb #25361 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 70976

Cat. # Size Qty. Price
70976S 1 Kit
10 assays
$891

Product Information

Storage

All components in this kit are stable for 12 months when stored at the recommended temperature.

Protocol

Product Description

The Human Anti-CD3/CD28 T Cell Activation Kit provides the necessary antibodies to induce human T cell activation and proliferation using the soluble stimulation protocol.

Specificity / Sensitivity

The Human Anti-CD3/CD28 T Cell Activation Kit is used for the stimulation of human T cells following the soluble stimulation protocol.

Background

T cells are activated by signaling through ITAM (immunoreceptor tyrosine-based activation motif)-containing CD3 signaling chains that associate with the T cell receptor (1). Co-stimulation through CD28 on T cells provides an additional signal required for effective T cell activation (2). Signaling through both CD3 and CD28 can be initiated using the agonist antibodies Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) and Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free), respectively. If the agonist antibodies are used in the soluble stimulation protocol, cross-linking of their Fc domains using Goat Anti-Mouse Kappa Light Chain, F(ab')2 Antibody (Low Endotoxin, Azide-free) is required for optimal stimulation.
For Research Use Only. Not For Use In Diagnostic Procedures.
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