Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 |
Jurkat |
Calyculin A (100 nM for 30 min) |
|
Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 |
Jurkat |
Calyculin A (100 nM for 30 min) |
|
Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb #13443 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Ser65) Antibody #9451 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Thr37/46) Antibody #9459 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb #13396 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-4E-BP1 (Thr70) Antibody #9455 |
MCF7 |
-AA/+Insulin (100 nM for 30 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-5-Lipoxygenase (Ser271) Antibody #3748 |
Transfected† |
Transfect cells with a construct overexpressing human 5-lipoxygenase |
|
Phospho-5-Lipoxygenase (Ser663) Antibody #3749 |
Transfected† |
Transfect cells with a construct overexpressing human 5-lipoxygenase |
|
Phospho-53BP1 (Ser1618) (D4H11) Rabbit mAb #6209 |
HeLa |
Thymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-53BP1 (Ser1778) Antibody #2675 |
HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-53BP1 (Ser25/29) Antibody #2674 |
HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-53BP1 (Thr543) Antibody #3428 |
HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Ack1 (Tyr284) Antibody #3138 |
Transfected† |
Transfect with a construct overexpressing human Ack1 |
|
Phospho-Afadin (Ser1718) Antibody #5485 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
|
Akt (pan) (C67E7) Rabbit mAb #4691 |
HeLa, NIH/3T3, C6 |
Untreated |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Ser473) (736E11) Rabbit mAb #3787 |
|
|
SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 |
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (APC Conjugate) #11962 |
Jurkat |
Calyculin A (100 nM for 30 min) |
|
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Ser473) Antibody #9271 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb #13038 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr308) Antibody #9275 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt (Thr450) (D5G4) Rabbit mAb #12178 |
NIH/3T3 |
hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-Akt (Thr450) Antibody #9267 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Akt Antibody #9272 |
HeLa, NIH/3T3, C6 |
Untreated |
Akt Control Cell Extracts #9273 |
Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb #10001 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-(Ser/Thr) Akt Substrate Antibody #9611 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt1 (Ser129) (D4P7F) Rabbit mAb #13461 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific) #9018 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific) #8599 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
ALK (D5F3®) XP® Rabbit mAb #3633 |
SUP-M2 |
Untreated |
|
Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb #12127 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1078) Antibody #4144 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb #6962 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1096) Antibody #4143 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb #6941 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1278) Antibody #3710 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1278/1282/1283) Antibody #3983 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1282/1283) (D39B2) Rabbit mAb #9687 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1282/1283) Antibody #3714 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb #14678 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1586) Antibody #3343 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-ALK (Tyr1604) Antibody #3341 |
KARPAS-299 |
Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) |
|
Phospho-AML1 (Ser249) Antibody #4327 |
A-431 |
TPA (80 nM, overnight) |
|
Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb #8084 |
Rat brain, mouse brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody #3921 |
Rat brain |
Ischemia (15 min) followed by reperfusion (4 hr) |
|
Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody #4027 |
Rat brain |
Ischemia (15 min) followed by reperfusion (4 hr) |
|
Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb #5759 |
C2C12 |
Serum-starve 30 min |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα (Thr172) Antibody #2531 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 |
293 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα1 (Ser485) Antibody #4184 |
C6 |
Serum-starve overnight, Oligomycin (0.5 μM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody #4185 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKβ1 (Ser108) Antibody #4181 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-AMPKβ1 (Ser182) Antibody #4186 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Androgen Receptor (D6F11) XP® Rabbit mAb #5153 |
LNCaP |
Untreated |
|
Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb #7399 |
HeLa |
H2O2 (4 mM for 30 min) |
|
Phospho-AP2M1 (Thr156) Antibody #3843 |
HeLa |
H2O2 (4 mM for 30 min) |
|
Phospho-APP (Thr668) (D90B8) Rabbit mAb #6986 |
HeLa |
Nocadazole (1 µg/ml for 18 hr) |
|
Phospho-APP (Thr668) Antibody #3823 |
HeLa |
Nocadazole (1 µg/ml for 18 hr) |
|
Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Arginase-1 (D4E3M™) XP® Rabbit mAb #93668 |
Mouse liver, rat liver |
Untreated |
|
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617 |
HeLa, COS |
Untreated |
|
Phospho-AS160 (Ser318) (D3D11) Rabbit mAb #8619 |
HeLa |
Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) |
|
Phospho-AS160 (Ser588) (D8E4) Rabbit mAb #8730 |
HeLa |
Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) |
|
Phospho-AS160 (Thr642) (D27E6) Rabbit mAb #8881 |
HeLa |
Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) |
|
Phospho-AS160 (Thr642) Antibody #4288 |
HeLa |
Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) |
|
Phospho-ASK1 (Ser967) Antibody #3764 |
Transfected† |
Transfect with a construct overexpressing human ASK1 |
|
Phospho-ASK1 (Thr845) Antibody #3765 |
Transfected† |
Transfect with a construct overexpressing human ASK1 |
|
Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb #3505 |
HeLa, C6, COS |
Untreated |
|
Phospho-ATF-2 (Thr69)/ATF-7 (Thr51) (E3D6B) Rabbit mAb #44167 |
NIH/3T3 |
Anisomycin (25 μg/ml for 30 min) |
ATF-2 Control Cell Extracts #9223 |
Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit mAb #61584 |
NIH/3T3 |
Anisomycin (25 μg/ml for 30 min) |
ATF-2 Control Cell Extracts #9223 |
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) (E4A5G) XP® Rabbit mAb #27934 |
NIH/3T3 |
Anisomycin (25 μg/ml for 30 min) |
ATF-2 Control Cell Extracts #9223 |
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Antibody #24329 |
NIH/3T3 |
Anisomycin (25 μg/ml for 30 min) |
ATF-2 Control Cell Extracts #9223 |
ATF-4 (D4B8) Rabbit mAb #11815 |
293, NIH/3T3, C6 |
Tunicamycin (2 μg/ml for 8 hr) |
|
Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb #13050 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-ATM (Ser1981) (D6H9) Rabbit mAb #5883 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909 |
3T3 |
UV‡ (50 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb #6966 |
3T3 |
UV‡ (50 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb #9607 |
3T3 |
UV‡ (50 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-(Ser/Thr) ATM/ATR Substrate Antibody #2851 |
293 |
UV‡ (50 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (50 ng/ml for 10 min) |
|
Phospho-ATR (Ser428) Antibody #2853 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-ATRIP (Ser224) Antibody #5161 |
293, HeLa |
Untreated |
|
Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr) |
|
Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr) |
|
Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724 |
NCI-H1299 |
Gas6 (400 ng/ml for 5 min) |
|
Phospho-B-Raf (Ser445) Antibody #2696 |
HeLa |
Serum-starve overnight, TPA (200 nM for 30 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-BACH1/BRIP1 (Thr1133) Antibody #11983 |
293 |
Untreated |
|
Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284 |
COS |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Bad (Ser112) (7E11) Mouse mAb #9296 |
COS |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Bad (Ser112) Antibody #9291 |
COS |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Bad (Ser136) (185D10) Rabbit mAb #5286 |
Transfected† |
Transfect with a construct overexpressing mouse or monkey Bad, serum-starve overnight, TPA (200 nM, 30 min) |
|
Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366 |
OVCAR-8 |
Untreated |
|
Phospho-Bad (Ser136) Antibody #9295 |
Transfected† |
Transfect with a construct overexpressing mouse Bad, serum-starve overnight, hPDGF-BB (100 ng/ml for 30 min) |
|
Phospho-Bad (Ser155) Antibody #9297 |
Transfected† |
Transfect with a construct overexpressing mouse Bad, serum-starve overnight, hPDGF-BB (100 ng/ml for 30 min) |
|
Phospho-BAP1 (Ser592) Antibody #9373 |
HeLa |
Serum-starve overnight, UV‡ (50 mJ/cm2, 1 hr recovery) |
|
Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827 |
Jurkat |
Paclitaxel (1 μM for 20 hr) |
|
Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875 |
RL-7 |
Paclitaxel (50 nM for 20 hr) |
|
Phospho-BCL9L (Ser915) Antibody #13325 |
MCF7 |
TPA (200 nM for 30 min) |
|
Phospho-Bcr (Tyr177) Antibody #3901 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-Beclin-1 (Ser15) (D4B7R) Rabbit mAb #84966 |
Transfected† |
Cotransfect with constructs overexpressing human or mouse Beclin-1 and ULK1 |
|
Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb #14717 |
A172 |
CCCP (100 µM for 2 hr) |
|
Phospho-Bim (Ser69) (D7E11) Rabbit mAb #4585 |
Raji, A20 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Bim (Ser69) Antibody #4581 |
Raji, A20 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Bim (Ser77) (D4H12) Rabbit mAb #12433 |
Raji, A20 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-BLNK (Tyr96) Antibody #3601 |
Ramos |
Anti-IgM (12 µg/ml for 2 min) |
|
Phospho-BMAL1 (Ser42) Antibody #13936 |
Mouse brain, rat brain |
Untreated |
|
Phospho-BRCA1 (Ser1524) Antibody #9009 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Btk (Ser180) (3D3) Mouse mAb #3537 |
THP-1 |
Serum-starve overnight, H2O2 (2 mM for 2 min) |
|
Phospho-Btk (Tyr223) Antibody #5082 |
Ramos |
Anti-IgM (12 μg/ml for 10 min) |
|
Phospho-c-Abl (Thr735) Antibody #2864 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb #3009 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Abl (Tyr245) Antibody #2861 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb #3098 |
K-562 |
Untreated (K-562 cell line contains Bcr-Abl fusion) |
|
Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb #8869 |
Jurkat |
Pervanadate (1 mM for 30 min) |
|
Phospho-c-Cbl (Tyr731) Antibody #3554 |
K-562 |
Untreated |
|
Phospho-c-Cbl (Tyr774) Antibody #3555 |
K-562 |
Untreated |
|
c-Fos (9F6) Rabbit mAb #2250 |
HeLa |
Serum-starve overnight, TPA (400 nM for 4 hr) |
|
Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb #5348 |
HeLa |
Serum-starve overnight, TPA (400 nM for 4 hr) |
|
Phospho-c-Jun (Ser243) Antibody #2994 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Ser63) (54B3) Rabbit mAb #2361 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Ser63) II Antibody #9261 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Ser73) Antibody #9164 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Thr91) Antibody #2303 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Jun (Thr93) Antibody #2993 |
NIH/3T3 |
Untreated |
c-Jun Control Cell Extracts #9263 |
Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073 |
NCI-H526 |
Serum-starve overnight, hSCF (100 ng/ml for 5 min) |
|
Phospho-c-Kit (Tyr719) Antibody #3391 |
NCI-H526 |
Serum-starve overnight, hSCF (100 ng/ml for 5 min) |
|
Phospho-c-Myc (Ser62) (E1J4K) Rabbit mAb #13748 |
HCT 116 |
MG-132 (10 µM, overnight) |
|
Phospho-c-Raf (Ser259) Antibody #9421 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-c-Raf (Ser289/296/301) Antibody #9431 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-c-Raf (Ser296) Antibody #9423 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-C/EBPbeta (Thr235) Antibody #3084 |
3T3-L1 |
Differentiated into adipocytes for 6 days, serum-starve overnight, insulin (100 nM for 10 min) |
|
Phospho-C/EBPα (Ser21) Antibody #2841 |
3T3-L1 |
Differentiated into adipocytes for 6 days, serum-starve overnight, insulin (100 nM for 45 min) |
|
Phospho-C/EBPα (Thr222/226) Antibody #2844 |
U-937 |
Untreated |
|
Phospho-CAD (Ser1859) Antibody #12662 |
HeLa, NIH/3T3 |
Untreated |
|
Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb #12716 |
Mouse brain, rat brain |
Untreated |
|
Phospho-CaMKII (Tyr231) Antibody #3356 |
Rat brain |
Ischemia (15 min) followed by reperfusion (4 hr) |
|
Phospho-CaMKK2 (Ser511) Antibody #12818 |
Mouse brain, rat brain |
Untreated |
|
Phospho-CARD11 (Ser652) Antibody #5189 |
Jurkat |
TPA (40 nM) + calcium ionophore A23187 (2 μM for 15 min) |
|
Caspase-3 Antibody #9662 |
HeLa, NIH/3T3, C6 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Caspase-3 Control Cell Extracts #9663 |
Phospho-Catenin δ-1 (Ser252) Antibody #8477 |
A-431 |
Untreated |
|
Phospho-Catenin δ-1 (Ser320) Antibody #8016 |
A-431 |
Untreated |
|
Phospho-Catenin δ-1 (Tyr228) Antibody #2911 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 15 min) |
|
Phospho-Catenin δ-1 (Tyr904) Antibody #2910 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 15 min) |
|
Phospho-Caveolin-1 (Tyr14) Antibody #3251 |
HeLa |
Serum-starve overnight, H2O2 (500 μM for 15 min) |
|
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771 |
HeLa, A431 |
Untreated |
|
Phospho-CD19 (Tyr531) Antibody #3571 |
Ramos |
Anti-IgM (12 μg/ml for 2 min) |
|
Phospho-CD79A (Tyr182) Antibody #5173 |
Ramos |
Anti-IgM (12 μg/ml for 2 min) |
|
Phospho-cdc2 (Thr14) Antibody #2543 |
293 |
Hydroxyurea (10 mM for 16 hr) |
|
Phospho-cdc2 (Thr161) Antibody #9114 |
293 |
Hydroxyurea (10 mM for 16 hr) |
|
Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539 |
HeLa |
Hydroxyurea (10 mM for 20 hr) |
|
Phospho-cdc2 (Tyr15) Antibody #9111 |
HeLa |
Hydroxyurea (10 mM for 20 hr) |
|
Phospho-CDC20 (Ser51) Antibody #8038 |
HeLa, 293 |
Untreated |
|
Phospho-cdc25C (Ser198) Antibody #9529 |
Transfected† |
Transfect with a construct overexpressing human cdc25C |
|
Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 |
HT-29, HeLa |
Nocadazole (100 ng/ml for 18 hr) - treatment diminishes signal |
|
Phospho-cdc25C (Ser216) Antibody #9528 |
HT-29, HeLa |
Nocadazole (100 ng/ml for 18 hr) - treatment diminishes signal |
|
Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb #12028 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-cdc25C (Thr48) Antibody #9527 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-CDC37 (Ser13) (D8P8F) Rabbit mAb #13248 |
293, C2C12, NIH/3T3 |
Untreated |
|
Phospho-CDCP1 (Tyr707) Antibody #13111 |
ZR-75 |
Cub1 Antibody (0.5µg/ml for 1 hr) |
|
Phospho-CDCP1 (Tyr734) Antibody #9050 |
HCT 116 |
Cub1 Antibody (0.5µg/ml for 1 hr) |
|
Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit mAb #14965 |
DLD-1 |
Cub1 Antibody (0.5µg/ml for 1 hr) |
|
Phospho-CDCP1 (Tyr806) Antibody #13024 |
ZR-75 |
Cub1 Antibody (0.5µg/ml for 1 hr) |
|
Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb #14371 |
HCT 116 |
Thymidine (2 mM for 18 hr) followed by Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-CDK2 (Thr160) Antibody #2561 |
HeLa |
Hydroxyurea (10 mM for 18 hr) |
|
Phospho-CDK9 (Thr186) Antibody #2549 |
HeLa |
Untreated |
|
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 |
HeLa |
Nocadazole (1 μg/ml for 12 hr) |
|
Phospho-CENP-A (Ser7) Antibody #2187 |
HT-29 |
Paclitaxel (100 nM overnight) |
|
Phospho-Chk1 (Ser280) Antibody #2347 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser296) (D3O9F) Rabbit mAb #90178 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser296) Antibody #2349 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb #12302 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser317) (D7H2) Rabbit mAb #8191 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser317) Antibody #2344 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk1 (Ser345) Antibody #2341 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk2 (Ser19) Antibody #2666 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk2 (Ser33/35) Antibody #2665 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk2 (Ser516) Antibody #2669 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-Chk2 (Thr68) Antibody #2661 |
293, HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
CHOP (L63F7) Mouse mAb #2895 |
C2C12 |
Thapsigargin (300 nM for 2 hr) |
CHOP Control Cell Extracts #33263 |
Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab™ Rabbit mAb mix #8738 |
HeLa |
Calyculin A (10 nM for 30 min) |
|
Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab™ Rabbit mAb mix #8698 |
HeLa |
Staurosporine (1 µM for 3 hr) |
|
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199 |
THP-1 |
TPA (80 nM overnight) + LPS (1 µg/ml for 8 hr) |
|
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
Caspase-3 Control Cell Extracts #9663 |
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (PE Conjugate) #12768 |
Jurkat |
Etoposide (25 µM for 5 hr) |
|
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579 |
Jurkat |
Etoposide (25 µM for 5 hr) |
|
Cleaved Caspase-3 (Asp175) Antibody #9661 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
Caspase-3 Control Cell Extracts #9663 |
Cleaved Caspase-6 (Asp162) Antibody #9761 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb #8438 |
Jurkat |
Etoposide (25 µM for 5 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-7 (Asp198) Antibody #9491 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
Caspase-3 Control Cell Extracts #9663 |
Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748 |
THP-1 |
Cycloheximide (10 μg/ml) + hTNF-α (20 ng/ml, overnight) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592 |
CTLL-2 |
Cycloheximide (10 µg/ml, overnight) + mTNF-α (20 ng/ml for 4 hr) |
|
Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) #9429 |
CTLL-2 |
Cycloheximide (10 µg/ml, overnight) + mTNF-α (20 ng/ml for 4 hr) |
|
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496 |
THP-1 |
Cycloheximide (10 μg/ml) + hTNF-α (20 ng/ml, overnight) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-9 (Asp315) Antibody (Human Specific) #9505 |
Jurkat |
Etoposide (25 μM for 5 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 |
HeLa |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-9 (Asp330) Antibody (Human Specific) #9501 |
Jurkat |
Etoposide (25 μM for 5 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) #9509 |
L-929 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) #9507 |
C6 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved DFF45 (Asp224) Antibody #9731 |
Jurkat |
Etoposide (25 µM for 5 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved Drosophila Dcp-1 (Asp216) Antibody #9578 |
S2 |
Actinomycin D (1 µM for 5 hr) |
|
Cleaved Drosophila ICE (drICE) (Asp230) Antibody #9478 |
S2 |
Actinomycin D (1 µM for 5 hr) |
|
Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb #8068 |
HeLa |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb #12576 |
NTERA-2 |
Retinoic acid (10 µM for 3 days) |
|
Cleaved Lamin A (Asp230) Antibody #2031 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
|
Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
|
Cleaved Lamin A (Small Subunit) Antibody #2035 |
HeLa |
Serum-starve overnight, staurosporine (1 μM for 3 hr) |
|
Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 |
MOLT-4, Jurkat |
Untreated |
|
Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546 |
HeLa |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548 |
L-929 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 |
HeLa |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved PARP (Asp214) Antibody (Human Specific) #9541 |
HeLa |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544 |
L-929 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved PARP (Asp214) Antibody (Rat Specific) #9545 |
C6 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
|
Cleaved α-Fodrin (Asp1185) Antibody #2121 |
Jurkat |
Etoposide (25 μM for 5hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 |
C2C12 |
Serum-starve overnight, AICAR (0.5 mM for 30 min) |
AMPK Control Cell Extracts #9158 |
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313 |
HeLa, NIH/3T3, C2C12 |
Untreated |
|
Phospho-Cofilin (Ser3) Antibody #3311 |
HeLa, NIH/3T3, C2C12 |
Untreated |
|
Phospho-Connexin 43 (Ser368) Antibody #3511 |
COS-7, C6 |
TPA (0.2μM for 30 min) |
|
Phospho-Cortactin (Tyr421) Antibody #4569 |
HeLa |
Serum-starve overnight, H2O2 (2 nM for 2 min) |
|
Phospho-cPLA2 (Ser505) Antibody #2831 |
NIH/3T3 |
Untreated |
|
CREB (48H2) Rabbit mAb #9197 |
SK-N-MC, NIH/3T3, C6 |
Untreated |
CREB Control Cell Extracts #9193 |
Phospho-CREB (Ser133) (1B6) Mouse mAb #9196 |
SK-N-MC |
Forskolin (30 µM) + IBMX (0.5 mM for 30 min) |
CREB Control Cell Extracts #9193 |
Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 |
SK-N-MC |
Forskolin (30 µM) + IBMX (0.5 mM for 30 min) |
CREB Control Cell Extracts #9193 |
Phospho-CrkII (Tyr221) Antibody #3491 |
K-562 |
Untreated |
|
Phospho-CrkL (Tyr207) Antibody #3181 |
K-562 |
Untreated |
|
Phospho-CRMP-2 (Thr514) Antibody #9397 |
Rat brain, mouse brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-CTDSPL2 (Ser104) Antibody #5294 |
HeLa |
Untreated |
|
Phospho-Cyclin B1 (Ser116) Antibody #13495 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb #4133 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-Cyclin B1 (Ser147) Antibody #4131 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300 |
HT-1080 |
MG-132 (10 µM for 4 hr) |
|
Phospho-Cyclin E1 (Thr62) Antibody #4136 |
MCF7 |
Hydroxyurea (10 mM, overnight) |
|
Phospho-CYLD (Ser418) Antibody #4500 |
293 |
hTNF-α (20 ng/ml for 20 min) |
|
Phospho-Dab1 (Tyr220) Antibody #3327 |
Transfected† |
Cotransfect with constructs overexpressing human Dab1 and Fyn |
|
Phospho-Dab1 (Tyr232) Antibody #3325 |
Transfected† |
Cotransfect with constructs overexpressing human Dab1 and Fyn |
|
Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb #13703 |
Ramos, Daudi |
Anti-IgM (12 μg/ml for 10 min) |
|
Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb #3401 |
Mouse brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb #12438 |
MKN-45 |
Forskolin (30 µM for 20 min) |
|
Phospho-DARPP-32 (Thr75) Antibody #2301 |
Rat brain, mouse brain, SHSY5Y, Neuro2A |
PKA (12,500 U/100 μl for 1 hr) |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-DBC1 (Thr454) Antibody #4880 |
293 |
UV‡ (100 mJ/cm2, 4 hr recovery) |
|
Phospho-DDR1 (Tyr513) (E1N8F) Rabbit mAb #14531 |
T47-D |
Collagen (20 μg/ml for 18 hr) |
|
Phospho-DDR1 (Tyr792) Antibody #11994 |
T47-D |
Collagen (15 μg/ml for 4hr) |
|
Phospho-Desmoplakin (Ser165/166) Antibody #5885 |
A-431, Calu |
Untreated |
|
Di-Methyl Lysine Motif [dme-K] Rabbit mAb #14117 |
HeLa |
Adenosine-2', 3'-dialdehyde (AdOx, 100 µM for 24 hr) |
|
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb (HRP Conjugate) #14040 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb #2901 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb #5427 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb #4658 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H3 (Lys9) Antibody #9753 |
NIH/3T3 |
Untreated |
|
Di-Methyl-Histone H4 (Lys20) Antibody #9759 |
NIH/3T3 |
Untreated |
|
Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb #5327 |
HeLa, NIH/3T3 |
Untreated |
|
Phospho-DNAJC2/MPP11 (Ser47) Antibody #12397 |
293 |
Untreated |
|
Phospho-Doublecortin (Ser297) Antibody #4605 |
Fetal rat brain |
Untreated |
|
Phospho-Doublecortin (Ser334) (D11B10) Rabbit mAb #14053 |
Fetal rat brain |
Untreated |
|
Phospho-Doublecortin (Ser334) Antibody #3453 |
Rat brain, mouse brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-DR6 (Ser562) Antibody #7616 |
A-431 |
hEGF (100 ng/ml for 30 min) |
|
Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody #9209 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb #4494 |
HeLa |
Nocadazole (100 mg/ml for 17 hr) |
|
Phospho-DRP1 (Ser616) Antibody #3455 |
HeLa |
Nocadazole (100 mg/ml for 17 hr) |
|
Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb #6319 |
PC-12 |
Forskolin (20 μM for 1 hr) |
|
Phospho-DRP1 (Ser637) Antibody #4867 |
PC-12 |
PKA (12,500 U/100 μl for 1 hr) |
|
Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb #2857 |
HeLa |
ALLN (50 µM for 5–6 hr) |
|
DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 |
Transfected† |
Transfect with a construct overexpressing a DYKDDDDK-tagged protein |
|
E-Cadherin (24E10) Rabbit mAb #3195 |
MCF7 |
Untreated |
|
Phospho-eEF2 (Thr56) Antibody #2331 |
C6 |
Forskolin (10 µM for 1 hr) |
|
Phospho-eEF2k (Ser366) Antibody #3691 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
EGF Receptor (D38B1) XP® Rabbit mAb #4267 |
HeLa,A-431, mIMCD-3 |
Untreated |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Ser1046/1047) Antibody #2238 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb #8808 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Thr669) Antibody #3056 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Thr678) Rabbit Ab #14343 |
A549 |
TPA (200 nM, 15 min) |
|
Phospho-EGF Receptor (Tyr1045) Antibody #2237 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb #2236 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1068) Antibody #2234 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1086) Antibody #2220 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1148) Antibody #4404 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr845) (D63B4) Rabbit mAb #6963 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr845) Antibody #2231 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr992) Antibody #2235 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb #2641 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
EGF Receptor Control Cell Extracts #5634 |
Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 |
HeLa |
Thapsigargin (300 nM for 30 min) |
|
Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 |
HeLa |
Thapsigargin (300 nM for 30 min) |
|
Phospho-eIF2α (Ser51) Antibody #9721 |
HeLa |
Thapsigargin (300 nM for 30 min) |
|
Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb #8151 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-eIF4B (Ser406) Antibody #5399 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-eIF4B (Ser422) Antibody #3591 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-eIF4E (Ser209) Antibody #9741 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-eIF4G (Ser1108) Antibody #2441 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-Elk-1 (Ser383) (2B1) Mouse mAb #9186 |
Recombinant protein |
GST-Elk1 fusion protein phosphorylated in vitro by Erk1/2 |
Elk-1 Control Proteins #9183 |
Phospho-Elk-1 (Ser383) Antibody #9181 |
Recombinant protein |
GST-Elk1 fusion protein phosphorylated in vitro by Erk1/2 |
Elk-1 Control Proteins #9183 |
Phospho-eNOS (Ser113) Antibody #9575 |
BAEC |
Untreated |
|
Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570 |
BAEC |
hVEGF165 (50 ng/ml for 5 min) |
|
Phospho-eNOS (Ser1177) Antibody #9571 |
BAEC |
hVEGF165 (50 ng/ml for 5 min) |
|
Phospho-eNOS (Thr495) Antibody #9574 |
BAEC |
Untreated, 3 days post-confluence |
|
Phospho-ENSA (Ser67)/ARPP19 (Ser62) Antibody #5240 |
HeLa |
Thymidine (2 mM for 16 hr) followed by Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb #6347 |
MDA-MB-231 |
Serum-starve overnight, FBS (10% for 5 min) |
|
Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb #12677 |
MDA-MB-231 |
EphrinA1-Fc ligand (100 ng/ml for 5 min) |
|
Phospho-EphA2 (Tyr594) Antibody #3970 |
MDA-MB-231 |
EphrinA5-Fc ligand (100 ng/ml for 5 min) |
|
Phospho-EphA2 (Tyr772) Antibody #8244 |
MDA-MB-231 |
EphrinA1-Fc ligand (100 ng/ml for 5 min) |
|
Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb #8862 |
MDA-MB-231 |
EphrinA5-Fc ligand (100 ng/ml for 5 min) |
|
Phospho-Erk5 (Thr218/Tyr220) Antibody #3371 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min); IP with ERK5 Antibody prior to western blot |
|
Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb #2511 |
MCF7 |
Serum-starve overnight, hEGF (100 ng/ml) + estradiol (100 nM for 30 min) |
|
Phospho-Etk (Tyr40) Antibody #3211 |
Jurkat |
Serum-starve overnight, anti-CD3 (1 μg/ml) + anti-CD28 (0.5 μg/ml for 10 min) |
|
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb #3726 |
PC-12, MCF7 |
Untreated |
|
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141 |
A431 |
Serum-starve overnight, hEGF (100 ng/ml for 10 min) |
|
Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb #5484 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 10 min) |
|
Phospho-Ezrin (Tyr353) Antibody #3144 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 10 min) |
|
Phospho-FADD (Ser194) Antibody (Human Specific) #2781 |
Jurkat, HeLa |
Nocadazole (1 μg/ml for 24 hr) |
|
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 |
A549 |
Untreated |
|
Phospho-FAK (Tyr397) Antibody #3283 |
A549 |
Untreated |
|
Phospho-FAK (Tyr576/577) Antibody #3281 |
A549 |
Untreated |
|
Phospho-FAK (Tyr925) Antibody #3284 |
A549 |
Untreated |
|
Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb #3476 |
A204 |
Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min) |
|
Phospho-FGF Receptor (Tyr653/654) Antibody #3471 |
A204 |
Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min) |
|
Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb #2544 |
Transfected† |
Transfect with a construct overexpressing human FGFR1 |
|
Phospho-Filamin A (Ser2152) Antibody #4761 |
293 |
Untreated |
|
Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464 |
SEM |
Untreated |
|
Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474 |
SEM |
Untreated |
|
Phospho-FLT3 (Tyr591) (54H1) Mouse mAb #3466 |
Transfected† |
Transfect with a construct overexpressing human or mouse FLT3 |
|
Phospho-FLT3 (Tyr591) Antibody #3461 |
SEM |
Untreated |
|
Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577 |
SEM |
Untreated |
|
Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463 |
SEM |
Untreated |
|
Phospho-FoxM1 (Ser35) Antibody #14170 |
ACHN |
Nocadazole (100 ng/ml for 18 hr) |
|
Phospho-FoxM1 (Thr600) (D9M6G) Rabbit mAb #14655 |
HeLa |
Nocadazole (100 ng/ml for 16 hr) |
|
Phospho-FoxO1 (Ser256) Antibody #9461 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FoxO1 (Ser319) Antibody #2486 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599 |
Jurkat, NIH/3T3, COS-7 |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb #13129 |
293T |
Untreated |
Akt Control Cell Extracts #9273 |
Phospho-FoxO3a (Ser253) Antibody #9466 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FoxO3a (Ser294) Antibody #5538 |
C2C12 |
Serum-starve overnight, H2O2 (4 mM for 30 min) |
|
Phospho-FoxO3a (Ser318/321) Antibody #9465 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb #8174 |
SH-SY5Y |
MG-132 (10 μM for 4 hr) |
|
Phospho-FoxO3a (Ser7) Antibody #14724 |
293T |
Untreated |
|
Phospho-FoxO4 (Ser193) Antibody #9471 |
HeLa |
Serum-starve overnight, 20% serum for 30 min |
Akt Control Cell Extracts #9273 |
Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841 |
HeLa |
Serum-starve overnight, TPA (400 nM for 4 hr) |
|
Phospho-FRA1 (Ser265) Antibody #3880 |
HeLa |
Serum-starve overnight, TPA (400 nM for 4 hr) |
|
Phospho-FRS2-α (Tyr196) Antibody #3864 |
293 |
Serum-starve overnight, acidic FGF (10ng/ml for 15 min) |
|
Phospho-FRS2-α (Tyr436) Antibody #3861 |
293 |
Serum-starve overnight, acidic FGF (10ng/ml for 15 min) |
|
Phospho-Gab1 (Tyr307) Antibody #3234 |
Hep G2 |
Serum-starve overnight, HGF (40 ng/ml for 10 min) |
|
Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb #3233 |
T-47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 15 min) |
|
Phospho-Gab1 (Tyr627) Antibody #3231 |
T-47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 15 min) |
|
Phospho-Gab1 (Tyr659) Antibody #12745 |
T-47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 15 min) |
|
Phospho-Gab2 (Ser159) Antibody #3884 |
T-47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 15 min) |
|
Phospho-Gab2 (Tyr452) (C33G1) Rabbit mAb #3882 |
T-47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 15 min) |
|
Phospho-Gab2 (Tyr452) Antibody #3881 |
H-4-II-E |
Serum-starve overnight, HGF (40 ng/ml for 10 min) |
|
GAPDH (14C10) Rabbit mAb #2118 |
HeLa, NIH/3T3, C6 |
Untreated |
|
GAPDH (D16H11) XP® Rabbit mAb #5174 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Phospho-GEF-H1 (Ser886) (E1L6D) Rabbit mAb #14143 |
293T, HT-29 |
Untreated |
|
Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb #11873 |
U-2 OS |
Untreated |
|
Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb #13167 |
HeLa |
Untreated |
|
Phospho-GKAP (Ser346) Antibody #13638 |
Mouse brain, rat brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 |
A549 |
Serum-starve overnight, dexamethasone (100 nM for 1 hr) |
|
Phospho-Glycogen Synthase (Ser641) Antibody #3891 |
NIH/3T3 |
Untreated |
|
Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb #11817 |
NIH/3T3 |
Serum-starve overnight, insulin (100 nM, 5 min) |
|
Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb #9322 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb #8452 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb #8566 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3α/β (Ser21/9) Antibody #9331 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3β (Ser9) (D2Y9Y) Mouse mAb #14630 |
PC-3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3β (Ser9) Antibody #9336 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 15 min) |
|
Phospho-GSK-3β (Thr390) Antibody #3548 |
HeLa |
Paclitaxel (100 nM/ml for 20 hr) |
|
HA-Tag (C29F4) Rabbit mAb #3724 |
Transfected† |
Transfect with a construct overexpressing a HA-tagged protein |
|
Phospho-HDAC3 (Ser424) Antibody #3815 |
NIH/3T3 |
Untreated |
|
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443 |
HeLa |
Serum-starve overnight, Pervanadate (1 mM for 15 min) |
|
Phospho-HDAC4 (Ser632)/HDAC5 (Ser498)/HDAC7 (Ser486) Antibody #3424 |
HeLa |
Serum-starve overnight, Pervanadate (1 mM for 15 min) |
|
Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb #6942 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb #2243 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody #2249 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER2/ErbB2 (Tyr1248) Antibody #2247 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody #2244 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER2/ErbB2 (Tyr877) Antibody #2241 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb #4561 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb #4784 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb #4791 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842 |
MCF7 |
hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb #14525 |
T47D |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757 |
MCF7 |
Serum-starve overnight, hNRG-1 (100 ng/ml for 5 min) |
|
Phospho-HER4/ErbB4 (Tyr984) Antibody #3790 |
MCF7 |
Serum-starve overnight, neuregulin (100 ng/ml for 5 min) |
|
HIF-1α (D1S7W) XP® Rabbit mAb #36169 |
HepG2 |
Cobalt chloride (100 μM for 4 hr) |
|
HIF-1α (D2U3T) Rabbit mAb #14179 |
HepG2 |
Cobalt chloride (100 μM for 4 hr) |
|
His-Tag (D3I1O) XP® Rabbit mAb #12698 |
Transfected† |
Transfect with a construct overexpressing a His-tagged protein |
|
Acetyl-Histone H2A (Lys5) Antibody #2576 |
NIH/3T3 |
TSA (400 nM, overnight) |
|
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 |
293 |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Histone H2A.X (Ser139) Antibody #2577 |
293 |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Histone H2A.X (Ser139/Tyr142) Antibody #5438 |
293 |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb #9861 |
NIH/3T3, HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H2B (Lys12) Antibody #5410 |
NIH/3T3, HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H2B (Lys15) (D8H1) XP® Rabbit mAb #9083 |
NIH/3T3, HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H2B (Lys20) Antibody #2571 |
NIH/3T3, HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H2B (Lys5) (D5H1S) XP® Rabbit mAb #12799 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H2B (Lys5) Antibody #2574 |
NIH/3T3, HeLa |
TSA (400 nM, overnight) |
|
Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb #6959 |
HL-60 |
Etoposide (50 μm for 6 hr) |
|
Histone H3 (D1H2) XP® Rabbit mAb #4499 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb #13998 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys18) Antibody #9675 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb #14932 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb (HRP Conjugate) #14056 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb #8173 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys27) Antibody #4353 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys56) Antibody #4243 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate) #14052 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H3 (Lys9/Lys14) Antibody #9677 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711 |
NIH/3T3 |
Serum-starve + TSA (400 nM, overnight), followed by 20% serum for 15 min, then 100 nM Calyculin A for 15 min |
|
Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 |
NIH/3T3, HeLa |
Serum-starve overnight, followed by 20% serum for 15 min, then Calyculin A (100 nM for 15 min) |
|
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 |
NIH/3T3, HeLa |
Serum-starve overnight, followed by 20% serum for 15 min, then Calyculin A (100 nM for 15 min) |
|
Phospho-Histone H3 (Ser10) Antibody #9701 |
NIH/3T3, HeLa |
Serum-starve overnight, followed by 20% serum for 15 min, then Calyculin A (100 nM for 15 min) |
|
Phospho-Histone H3 (Ser28) Antibody #9713 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-Histone H3 (Thr11) (C2A6) Rabbit mAb #9767 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-Histone H3 (Thr11) Antibody #9764 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-Histone H3 (Thr3) (D5G1I) Rabbit mAb #13576 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-Histone H3 (Thr3) Antibody #9714 |
NIH/3T3, HeLa |
Serum-starve overnight, followed by 20% serum for 15 min, then Calyculin A (100 nM for 15 min) |
|
Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb #13944 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H4 (Lys12) Antibody #2591 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb #13534 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb #8647 |
HeLa |
TSA (400 nM, overnight) |
|
Acetyl-Histone H4 (Lys8) Antibody #2594 |
HeLa |
TSA (400 nM, overnight) |
|
Phospho-HP1γ (Ser83) Antibody #2600 |
HeLa |
Forskolin (10 µM for 1 hr) |
|
Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714 |
Ramos |
Serum-starve overnight, anti-IgM antibody (12 μg/ml for 10 min) |
|
Phospho-HS1 (Tyr397) Antibody #4507 |
Ramos |
Serum-starve overnight, anti-IgM antibody (12 μg/ml for 10 min) |
|
Phospho-HSL (Ser563) Antibody #4139 |
3T3-L1 |
Differentiated into adipocytes for 4 days, Isoproterenol (10 µM for 20 min) |
|
Phospho-HSL (Ser565) Antibody #4137 |
3T3-L1 |
Differentiated into adipocytes for 4 days, Isoproterenol (10 µM for 20 min) |
|
Phospho-HSP27 (Ser15) Antibody #2404 |
HeLa |
Serum-starve overnight, UV‡ (60 mJ/cm2, 30 min recovery) |
|
Phospho-HSP27 (Ser78) Antibody #2405 |
HeLa |
Serum-starve overnight, UV‡ (60 mJ/cm2, 30 min recovery) |
|
Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb #9709 |
HeLa |
Anisomycin (25 μg/ml for 30 min) |
|
Phospho-HSP27 (Ser82) Antibody #2401 |
HeLa |
Serum-starve overnight, UV‡ (40 mJ/cm2, 30 min recovery) |
|
Phospho-HSP27 (Ser82) Antibody II #2406 |
HeLa |
Serum-starve overnight, UV‡ (40 mJ/cm2, 30 min recovery) |
|
Phospho-HSP90α (Thr5/7) Antibody #3488 |
HeLa |
Serum-starve overnight, UV‡ (40 mJ/cm2, 30 min recovery) |
|
Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021 |
293 |
Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) |
|
Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb #3918 |
293 |
Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) |
|
Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024 |
293 |
Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) |
|
Phospho-IGF-I Receptor β (Tyr1316) Antibody #6113 |
293 |
Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) |
|
Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb #4568 |
293 |
Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) |
|
Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb (PE Conjugate) #14938 |
HeLa |
hTNF-α (20 ng/ml) + Calyculin A (100 nM for 15 min) |
|
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-IKKα/β (Ser176/180) Antibody II #2694 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-IKKγ (Ser376) Antibody #2689 |
HeLa |
hTNF-α (20 ng/ml) + Calyculin A (50 nM for 5 min) |
|
Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766 |
THP-1 |
TPA (80 nM, overnight) + LPS (1 μg/ml for 15 min) |
|
IL-1β (3A6) Mouse mAb #12242 |
THP-1 |
TPA (80 nM, overnight) + LPS (100 ng/ml for 3+ hr) |
|
Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb #3026 |
Transfected† |
Transfect with a construct overexpressing human IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb #3023 |
Transfected† |
Transfect with a construct overexpressing human IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb #8548 |
Mouse brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-IP3 Receptor (Ser1756) Antibody #3760 |
Mouse brain, rat brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb #11927 |
KARPAS-299 |
hIL-1β (50 ng/ml for 15 min) |
|
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 |
RAW264.7 |
LPS (1 μg/ml for 2 hr) |
|
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (PE Conjugate) #83611 |
RAW264.7 |
LPS (1 μg/ml for 2 hr) |
|
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047 |
RAW264.7 |
LPS (1 μg/ml for 2 hr) |
|
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047 |
HT-29, A549 |
Poly(I:C) (2.5 μg/ml for 6 hr) |
|
Phospho-IRF-7 (Ser471/472) Antibody #5184 |
Transfected† |
Transfect with a construct overexpressing human IRF-7 |
|
Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb #12390 |
HT-29 |
hIFN-α1 (10 ng/ml overnight) and poly(I:C) (2.5 μg/ml for 7 hr) |
|
Phospho-IRS-1 (Ser1101) Antibody #2385 |
Differentiated C2C12 (myotubes) |
Serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491 |
MCF-7, C2C12 |
Serum-starve overnight, insulin (100 nM for 5 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-IRS-1 (Ser302) Antibody #2384 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-IRS-1 (Ser307) Antibody #2381 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
4E-BP1 Control Cell Extracts #51367 |
Phospho-IRS-1 (Ser332/336) Antibody #2580 |
Transfected† |
Transfect with a construct overexpressing rat IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) |
|
Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb #3193 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IRS-1 (Ser612) Antibody #2386 |
mIMCD-3 |
Serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IRS-1 (Ser636/639) Antibody #2388 |
MCF7 |
Serum-starve overnight, insulin (100 nM for 5 min) |
|
Phospho-IRS-1 (Ser789) Antibody #2389 |
Transfected† |
Transfect with a construct overexpressing rat IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) |
|
Phospho-IRS-1 (Tyr1222) Antibody #3066 |
Transfected† |
Transfect with a construct overexpressing rat IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) |
|
Phospho-IRS-1 (Tyr895) Antibody #3070 |
Transfected† |
Transfect with a construct overexpressing human IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) |
|
Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-IκBβ (Thr19/Ser23) Antibody (Human Specific) #4921 |
HeLa |
hTNF-α (20 ng/ml) + Calyculin A (50 nM for 5 min) |
|
Phospho-IκBε (Ser18/22) Antibody #4924 |
HeLa |
hTNF-α (20 ng/ml) + Calyculin A (50 nM for 5 min) |
|
Phospho-Jak1 (Tyr1022/1023) Antibody #3331 |
CTLL-2 |
Untreated |
|
Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb #4406 |
UT7 |
Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation |
|
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 |
UT7 |
Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation |
|
Phospho-Jak2 (Tyr1007/1008) Antibody #3771 |
UT7 |
Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation |
|
Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb #8082 |
UT7 |
Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation |
|
Phospho-Jak2 (Tyr221) Antibody #3774 |
CTLL-2 |
Untreated |
|
Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb #5031 |
NK-92 |
Cytokine-starve overnight, IL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth |
|
Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053 |
C6 |
Anisomycin (25 μg/ml for 20 min) |
p38 MAPK Control Cell Extracts #9213 |
Phospho-Keratin 17 (Ser44) Antibody #3519 |
HeLa |
Nocadazole (100 ng/ml for 4 hr) |
|
Phospho-KIF1B (Ser1487) Antibody #11993 |
PC-3 |
Untreated |
|
Phospho-KSR1 (Ser392) Antibody #4951 |
HeLa |
Untreated |
|
Phospho-Lamin A/C (Ser22) (D2B2E) XP® Rabbit mAb #13448 |
HeLa, NIH/3T3, C6 |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Lamin A/C (Ser22) Antibody #2026 |
THP-1 |
Nocadazole (1 μg/ml for 20 hr) |
|
Phospho-LAT (Tyr171) Antibody #3581 |
Jurkat |
Anti-CD3 (10 µg/ml for 2 min) |
|
Phospho-LAT (Tyr191) Antibody #3584 |
Jurkat |
Anti-CD3 (10 µg/ml for 2 min) |
|
Phospho-LATS1 (Ser909) Antibody #9157 |
HeLa |
TPA (200 nM for 30 min) |
|
Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654 |
HeLa |
Okadaic Acid (1 μM for 1 hr) |
|
LC3A/B (D3U4C) XP® Rabbit mAb #12741 |
HeLa, NIH/3T3, C6 |
Chloroquine (50 μM, overnight) |
LC3 Control Cell Extracts #11972 |
LC3B (D11) XP® Rabbit mAb #3868 |
HeLa |
Chloroquine (50 μM, overnight) |
LC3 Control Cell Extracts #11972 |
LC3B Antibody #2775 |
HeLa, NIH/3T3, C6 |
Chloroquine (50 μM, overnight) |
LC3 Control Cell Extracts #11972 |
Phospho-Lck (Tyr505) Antibody #2751 |
Jurkat |
Untreated |
|
Phospho-LCP1 (Tyr28) Antibody #5277 |
MOLT-15, MV-4-11 |
Untreated |
|
Phospho-LDHA (Tyr10) Antibody #8176 |
K562 |
Untreated |
|
Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841 |
Transfected† |
Transfect with a construct overexpressing human LIMK1 |
|
Phospho-LKB1 (Ser334) Antibody #3055 |
Transfected† |
Transfect with a construct overexpressing human LKB1 |
|
Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb #3482 |
293 |
TPA (200 nM for 30 min) |
|
Phospho-LKB1 (Thr189) Antibody #3054 |
Transfected† |
Transfect with a construct overexpressing human LKB1 |
|
Phospho-LRP6 (Ser1490) Antibody #2568 |
HeLa |
Conditioned media from L cells transfected with Wnt3a ligand (5 hr) |
|
Phospho-Lyn (Tyr507) Antibody #2731 |
Ramos |
Anti-IgM (12 μg/ml for 10 min) |
|
Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) #6952 |
COS |
TSA (0.4 µM for 18 hr) |
|
Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814 |
COS |
TSA (0.4 μM for 18 hr) |
|
Acetylated-Lysine Antibody #9441 |
COS |
TSA (0.4 μM for 18 hr) |
|
Acetylated-Lysine Mouse mAb (Ac-K-103) #9681 |
COS |
TSA (0.4 μM for 18 hr) |
|
Phospho-M-CSF Receptor (Tyr546) Antibody #3083 |
Transfected† |
Transfect with a construct overexpressing human or mouse CSFR1 |
|
Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb #12251 |
GDM1 |
hM-CSF (100 ng/ml for 10 min) |
|
Phospho-M-CSF Receptor (Tyr699) Antibody #3399 |
NKM-1 |
Untreated |
|
Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb #14591 |
GDM-1 |
Serum-starve overnight; hM-CSF 100 ng/ml for 5 min |
|
Phospho-M-CSF Receptor (Tyr708) Antibody #3080 |
Transfected† |
Transfect with a construct overexpressing human or mouse CSFR1 |
|
Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb #3155 |
NKM-1 |
Untreated |
|
Phospho-M-CSF Receptor (Tyr723) Antibody #3151 |
NKM-1 |
Untreated |
|
Phospho-M-CSF Receptor (Tyr809) Antibody #3154 |
NKM-1 |
Untreated |
|
Phospho-M-CSF Receptor (Tyr923) Antibody #3406 |
NKM-1 |
Untreated |
|
Malonyl-Lysine [Mal-K] MultiMab™ Rabbit mAb mix #14942 |
HepG2, mouse liver |
Untreated |
|
Phospho-MAP2 (Ser136) Antibody #4541 |
PC-12 |
Nocadazole (1 µg/ml for 16 hr) |
|
Phospho-MAP2 (Thr1620/1623) Antibody #4544 |
PC-12 |
Nocadazole (1 µg/ml for 16 hr) |
|
Phospho-MAPK Substrates Motif [PXpTP] MultiMab™ Rabbit mAb mix #14378 |
HCT 116 |
Thymidine (2 mM for 18 hr) + Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-MAPK Substrates Motif [PXpTP] Rabbit mAb #14378 |
HCT 116 |
Thymidine (2 mM for 18 hr) + Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 |
HeLa |
Nocadazole (1 μg/ml for 12 hr) |
|
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 |
HeLa |
Serum-starve overnight, UV‡ (40 mJ/cm2, 20 min recovery) |
|
Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 |
HeLa |
Serum-starve overnight, UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-MAPKAPK-2 (Thr334) Antibody #3041 |
HeLa |
Serum-starve overnight, UV‡ (40 mJ/cm2, 20 min recovery) |
|
Phospho-MARCKS (Ser152/156) Antibody #2741 |
HeLa, NIH/3T3 |
Serum-starve overnight, TPA (200 nM for 15 min) |
|
Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb #11992 |
HeLa, NIH/3T3, C6 |
Serum-starve overnight, TPA (200 nM for 15 min) |
|
Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb #8722 |
SK-N-MC |
TPA (200 nM for 15 min) |
|
Phospho-MARK Family (Activation Loop) Antibody #4836 |
Raji, BaF3 |
Untreated |
|
Phospho-Mcl-1 (Ser159/Thr163) Antibody #4579 |
NCI-H929 |
MG-132 (10 μM for 2 hr) |
|
Phospho-Mcl-1 (Ser64) Antibody #13297 |
HCT 116, KARPAS-299 |
MG-132 (10 μM, overnight) |
|
Phospho-Mcl-1 (Thr163) (D5M9D) Rabbit mAb #14765 |
Raji, KARPAS-299 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb #12958 |
HeLa, C2C12 |
Untreated |
|
Phospho-MCM2 (Ser139) Antibody #8861 |
HeLa, C2C12 |
Untreated |
|
Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb #12686 |
HeLa |
Nocadazole (100 ng/ml for 24 hr) |
|
Phospho-MDM2 (Ser166) Antibody #3521 |
A431 |
EGF (100 ng/ml for 5 mins) |
|
Phospho-MEF2A (Ser408) Antibody #9737 |
Transfected† |
Transfect with a construct overexpressing human MEF2A |
|
Phospho-MEK1 (Ser298) Antibody #9128 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-MEK1 (Thr286) Antibody #9127 |
HeLa |
Serum-starve overnight, Nocadazole (1 μg/ml for 16 hr) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-MEK 1/2 (Ser217/221) (41G9) Rabbit mAb #9154 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-MEK 1/2 (Ser217/221) Antibody #9121 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-MEK 1/2 (Ser221) (166F8) Rabbit mAb #2338 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-MERIT40 (Ser29) Antibody #12110 |
A-431 |
Untreated |
|
Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb #13281 |
PC-3 |
Untreated |
|
Phospho-Merlin (Ser518) Antibody #9163 |
PC-3 |
Untreated |
|
Phospho-Met (Tyr1003) (13D11) Rabbit mAb #3135 |
H-4-II-E |
Serum-starve overnight, EGF (40 ng/ml for 10 min) |
|
Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb #3129 |
H-4-II-E |
Serum-starve overnight, HGF (100 ng/ml for 5 min) |
|
Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077 |
H-4-II-E |
Serum-starve overnight, EGF (40 ng/ml for 10 min) |
|
Phospho-Met (Tyr1234/1235) Antibody #3126 |
H-4-II-E |
Serum-starve overnight, HGF (40 ng/ml for 10 min) |
|
Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133 |
H-4-II-E |
Serum-starve overnight, HGF (40 ng/ml for 10 min) |
|
Phospho-Met (Tyr1349) Antibody #3121 |
H-4-II-E |
Serum-starve overnight, HGF (40 ng/ml for 10 min) |
|
Methyl-Histone H3 (Arg2) Antibody #9707 |
HeLa |
Untreated |
|
Methyl-NF-κB p65 (Lys310) Antibody #13188 |
HT-1080, OVCAR8 |
Untreated |
|
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb #9236 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb #12280 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231 |
NIH/3T3 |
UV‡ (40 mJ/cm2, 30 min recovery) |
MKK3/MKK6 Control Cell Extracts #9233 |
Phospho-MKK7 (Ser271/Thr275) Antibody #4171 |
U-937 |
Sorbitol (400 mM for 15 min) |
|
Phospho-MLKL (Ser358) (D6H3V) Rabbit mAb #91689 |
HT-29 |
Z-VAD (20 μM for 30 min) followed by hTNF-α (20 ng/ml) + SM-164 (100 nM for 7 hr) |
|
Phospho-Mnk1 (Thr197/202) Antibody #2111 |
HeLa |
Anisomycin (25 µg/ml for 30 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb #8843 |
MCF7 |
H202 (2.5 mM for 30 min) |
|
Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb #8699 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb #14111 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys4) Antibody #9723 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb #12522 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys79) Antibody #9398 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb #14186 |
HeLa, NIH/3T3 |
Untreated |
|
Mono-Methyl-Histone H4 (Lys20) Antibody #9724 |
NIH/3T3 |
Untreated |
|
Phospho-Mre11 (Ser676) Antibody #4859 |
HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-MSK1 (Ser360) Antibody #9594 |
Transfected† |
Transfect with a construct overexpressing human MSK1 |
|
Phospho-MSK1 (Ser376) Antibody #9591 |
293 |
UV‡ (40 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-MSK1 (Thr581) Antibody #9595 |
HeLa |
Calyculin A (100 nM for 30 min) |
SAPK/JNK Control Cell Extracts #9253 |
mTOR (7C10) Rabbit mAb #2983 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-mTOR (Ser2448) Antibody #2971 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-mTOR (Ser2481) Antibody #2974 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Myc-Tag (9B11) Mouse mAb #2276 |
Transfected† |
Transfect with a construct overexpressing a Myc-tagged protein |
|
Phospho-Myosin IIa (Ser1943) (D7Z7T) Rabbit mAb #14611 |
293T, HeLa |
Untreated |
|
Phospho-Myosin IIa (Ser1943) Antibody #5026 |
293T, HeLa |
Untreated |
|
Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671 |
C2C12 |
Untreated |
|
Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675 |
C2C12 |
Untreated |
|
Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674 |
C2C12 |
Untreated |
|
Phospho-MYPT1 (Ser507) Antibody #3040 |
HeLa |
Serum-starve overnight, Calyculin A (100 nM for 30 min) |
|
Phospho-MYPT1 (Ser668) Antibody #3048 |
HeLa |
Serum-starve overnight, Calyculin A (100 nM for 30 min) |
|
Phospho-MYPT1 (Thr696) Antibody #5163 |
293, HeLa |
Untreated |
|
Phospho-MYPT1 (Thr853) Antibody #4563 |
293, HeLa |
Untreated |
|
Phospho-Myt1 (Ser83) Antibody #4281 |
HeLa, HT-29 |
Nocadazole (50 ng/ml for 20 hr) |
|
N-Cadherin (D4R1H) XP® Rabbit mAb #13116 |
A172 |
Untreated |
|
Phospho-Na,K-ATPase α1 (Ser16) Antibody #4020 |
PC-12 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Na,K-ATPase α1 (Ser23) Antibody #4006 |
PC-12 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb #13566 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 2 min) |
|
Phospho-Na,K-ATPase α1 (Tyr10) Antibody #3060 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
|
Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb #11899 |
Jurkat, LNCap |
Untreated |
|
Phospho-NDRG1 (Ser330) Antibody #3506 |
Jurkat, LNCap |
Untreated |
|
Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (HRP Conjugate) #8947 |
C2C12 |
Untreated |
|
Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482 |
C2C12 |
Untreated |
|
Phospho-NDRG1 (Thr346) Antibody #3217 |
C2C12 |
Untreated |
|
Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb #12146 |
SK-MEL-28 |
Untreated |
|
Phospho-NEDD4L (Ser448) Antibody #8063 |
SK-MEL-28 |
Untreated |
|
Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
NF-κB p65 (D14E12) XP® Rabbit mAb #8242 |
HeLa, NIH/3T3, C6 |
Untreated |
NF-κB Control Cell Extracts #9243 |
Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb #12629 |
Transfected† |
Cotransfect with constructs overexpressing human or mouse NF-κB p65 and CBP/p300 |
|
Acetyl-NF-κB p65 (Lys310) Antibody #3045 |
Transfected† |
Cotransfect with constructs overexpressing human or mouse NF-κB p65 and CBP/p300 |
|
Phospho-NF-κB p65 (Ser468) Antibody #3039 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb #3036 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb #13346 |
Differentiated THP-1 |
TPA (80 nM, overnight), LPS (1 μg/ml for 15 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-NF-κB p65 (Ser536) Antibody #3031 |
HeLa |
hTNF-α (20 ng/ml for 5 min) |
NF-κB Control Cell Extracts #9243 |
Phospho-NF-κB2 p100 (Ser866/870) Antibody #4810 |
Transfected† |
Cotransfect with constructs overexpressing human and mouse NIK and p100 |
|
NLRP3 (D4D8T) Rabbit mAb #15101 |
THP-1, J774A.1 |
Untreated |
|
Phospho-NMDAR1 (Ser890) Antibody #3381 |
Transfected† |
Transfect with a construct expressing human NMDAR1, TPA (100 nM for 5 min) |
|
Phospho-NMDAR1 (Ser896) Antibody #3384 |
Transfected† |
Transfect with a construct expressing human NMDAR1, TPA (100 nM for 5 min) |
|
Phospho-NMDAR1 (Ser897) Antibody #3385 |
Transfected† |
Transfect with a construct expressing human NMDAR1, Forskolin (30 μM for 20 min) |
|
Phospho-NMDAR2A (Tyr1246) Antibody #4206 |
Rat brain |
Untreated |
|
Phospho-NMDAR2B (Ser1284) Antibody #5355 |
Mouse brain, rat brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-NMDAR2B (Tyr1070) Antibody #4209 |
Rat brain |
Untreated |
|
Phospho-NMDAR2B (Tyr1472) Antibody #4208 |
Rat brain |
Ischemia (15 min) followed by reperfusion (4 hr) |
|
Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-NPM (Thr199) Antibody #3541 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-NPM (Thr95) Antibody #3517 |
HeLa |
Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-NuMA (Ser395) Antibody #3429 |
M059K |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-NuMA (Thr2055) Antibody #14951 |
HT-29 |
Thymidine (2 mM for 16 hr), add fresh media + Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off |
|
Phospho-Numb (Ser276) (D5C2) Rabbit mAb #9878 |
HeLa |
TPA (200 nM for 15-30 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-Numb (Ser276) Antibody #4140 |
HeLa |
TPA (200 nM for 15-30 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb #5095 |
Jurkat cells |
TPA (400 nM for 4 hr) |
|
Phospho-p130 Cas (Tyr165) Antibody #4015 |
NIH/3T3 |
Untreated |
|
Phospho-p130 Cas (Tyr249) Antibody #4014 |
NIH/3T3 |
Untreated |
|
Phospho-p130 Cas (Tyr410) Antibody #4011 |
NIH/3T3 |
Untreated |
|
p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 |
MCF7 |
Untreated |
|
Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb #4631 |
C6 |
Anisomycin (25 μg/ml for 30 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb #9216 |
C6 |
Anisomycin (25 μg/ml for 20 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 |
C6 |
Anisomycin (25 μg/ml for 20 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 |
C6 |
Anisomycin (25 μg/ml for 20 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 |
C6 |
Anisomycin (25 μg/ml for 30 min) |
p38 MAP Kinase Control Cell Extracts #9213 |
Phospho-p40phox (Thr154) Antibody #4311 |
THP-1 |
TPA (80 nM, overnight), LPS (1 μg/ml for 15 min) |
|
Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb #5726 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 |
HeLa, NIH/3T3, C6 |
Untreated |
p44/p42 MAPK (Erk1/2) Control Cell Extracts #9194 |
p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 |
HeLa, NIH/3T3, C6 |
Untreated |
p44/p42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (HRP Conjugate) #14474 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb #4377 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (HRP Conjugate) #8544 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 |
Jurkat |
TPA (200 nM for 10 min) |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
p44/42 MAPK (Erk1/2) Antibody #9102 |
HeLa, NIH/3T3, C6 |
Untreated |
p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
p53 (1C12) Mouse mAb #2524 |
HT-29, 293 |
Untreated |
|
Acetyl-p53 (Lys379) Antibody #2570 |
HT-29 |
Etoposide (25 µM, overnight) |
|
Acetyl-p53 (Lys382) Antibody #2525 |
HT-29 |
Etoposide (25 µM, overnight) |
|
Phospho-p53 (Ser15) (16G8) Mouse mAb #9286 |
HT-29 |
Etoposide (25 µM, overnight) |
|
Phospho-p53 (Ser15) (D4S1H) XP® Rabbit mAb (Rodent Specific) #12571 |
L-929 |
Etoposide (30 µM for 2 hr) |
|
Phospho-p53 (Ser15) Antibody #9284 |
HT-29 |
Etoposide (25 µM, overnight) |
|
Phospho-p53 (Ser20) Antibody #9287 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Ser315) Antibody #2528 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Ser33) Antibody #2526 |
HT-29 |
Nocadazole (50 ng/ml for 24–48 hr) |
|
Phospho-p53 (Ser37) Antibody #9289 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Ser392) Antibody #9281 |
293 |
Hydroxyurea (20 mM for 30 hr) |
|
Phospho-p53 (Ser46) Antibody #2521 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Ser6) Antibody #9285 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Ser9) Antibody #9288 |
293 |
UV‡ (50 mJ/cm2, 30 minute recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-p53 (Thr81) Antibody #2676 |
HT-29 |
Nocadazole (50 ng/ml for 24–48 hr) |
|
Phospho-p56Dok-2 (Tyr351) Antibody #3911 |
Transfected† |
Transfect with a construct overexpressing human p56Dok-2 |
|
Phospho-p57 Kip2 (Thr310) Antibody #2558 |
HeLa |
Dexamethasone (50 nM for 16 hr) |
|
Phospho-p63 (Ser160/162) Antibody #4981 |
ME-180 |
Nocadazole (50 ng/ml for 24 hr) |
|
Phospho-p70 S6 Kinase (Ser371) Antibody #9208 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-p70 S6 Kinase (Thr389) Antibody #9205 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody #9204 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-p73 (Tyr99) Antibody #4665 |
HT-1376 |
Pervanadate (1 mM for 20 min) |
|
Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb #11989 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb #12032 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Ser380) Antibody #9341 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Thr359/Ser363) Antibody #9344 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p90RSK (Thr573) Antibody #9346 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK 1/2 Control Cell Extracts #9160 |
Phospho-p95/NBS1 (Ser343) Antibody #3001 |
HeLa |
UV‡ (50 mJ/cm2, 2 hr recovery) |
|
Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606 |
Guinea pig neutrophils |
fMLP (1 µM for 30 sec) |
|
Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605 |
Guinea pig neutrophils |
fMLP (1 µM for 30 sec) |
|
Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601 |
Guinea pig neutrophils |
fMLP (1 µM for 30 sec) |
|
Phospho-PAK2 (Ser20) Antibody #2607 |
Guinea pig neutrophils |
fMLP (1 µM for 30 sec) |
|
Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody #3241 |
Guinea pig neutrophils |
fMLP (1 µM for 30 sec) |
|
Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb (HRP Conjugate) #6951 |
HeLa, NIH/3T3 |
Untreated |
|
Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb #4473 |
HeLa, NIH/3T3 |
Untreated |
|
Phospho-PAR-4 (Thr163) Antibody #2329 |
HeLa |
Calyculin A (100 nM for 5 min) |
|
PARP Antibody #9542 |
HeLa, NIH/3T3, C6 |
Serum-starve overnight, staurosporine (1 µM for 3 hr) |
Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
Phospho-Paxillin (Tyr118) Antibody #2541 |
C2C12, NIH/3T3, A-431, C6 |
Untreated |
|
Phospho-PBK/TOPK (Thr9) Antibody #4941 |
HT-29 |
Nocadazole (50 ng/ml for 20 hr) |
|
PD-L1 (E1L3N®) XP® Rabbit mAb #13684 |
SUP-M2 |
Untreated |
|
Phospho-PDGF Receptor α (Tyr1018) Antibody #4547 |
NIH/3T3 |
Serum-starve overnight, hPDGF-AA (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992 |
NIH/3T3 |
Serum-starve overnight, hPDGF-AA (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor α (Tyr762) (D9B1N) Rabbit mAb #24188 |
NCI-H1703 |
hPDGF-AA (100 ng/ml for 10 min) |
|
Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb #3170 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb #2227 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb #3168 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb #3166 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb #4549 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr751) Antibody #3161 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438 |
HeLa, NIH/3T3 |
Untreated |
|
Phospho-PDK1 (Ser241) Antibody #3061 |
HeLa |
Untreated |
|
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-PEA-15 (Ser104) Antibody #2776 |
C6 |
TPA (200 nM for 30 min) |
|
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179 |
C6 |
Thapsigargin (1 μM for 20 min) |
|
Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb #13064 |
HeLa |
Serum-starve overnight; hIGF-1 (100 ng/ml for 5 min) |
|
Phospho-(Ser/Thr) Phe Antibody #9631 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Phospholamban (Ser16/Thr17) Antibody #8496 |
Spontaneous hypertensive (SHR) rat heart |
Untreated |
|
Phospho-PI3 Kinase Class III (Ser249) Antibody #13857 |
Transfected† |
Transfect with a construct overexpressing human PI3 Kinase Class III and ULK1 |
|
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228 |
NIH/3T3-Src |
Untreated |
|
Phospho-PKA C (Thr197) (D45D3) Rabbit mAb #5661 |
NIH/3T3 |
Untreated |
|
Phospho-PKA C (Thr197) Antibody #4781 |
NIH/3T3 |
Untreated |
|
Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb #9624 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-(Ser/Thr) PKA Substrate Antibody #9621 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-PKC (pan) (gamma Thr514) (D6Y3D) Rabbit mAb #38938 |
HeLa, mouse brain |
Untreated |
|
Phospho-PKC (pan) (gamma Thr514) Antibody #9379 |
HeLa |
Untreated |
|
Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb #2060 |
HeLa |
Untreated |
|
Phospho-PKC (pan) (βII Ser660) Antibody #9371 |
HeLa |
Untreated |
|
Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb #6967 |
HeLa |
TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-(Ser) PKC Substrate Antibody #2261 |
HeLa |
TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PKCdelta (Tyr311) Antibody #2055 |
U937 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-PKCα/β II (Thr638/641) Antibody #9375 |
HeLa |
Untreated |
|
Phospho-PKCδ (Ser359) (D2X1P) Rabbit mAb #14787 |
OVCAR8, HeLa |
Untreated |
|
Phospho-PKCδ (Thr505) Antibody #9374 |
U937 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-PKCδ/θ (Ser643/676) Antibody #9376 |
HeLa |
Untreated |
|
Phospho-PKCζ/λ (Thr410/403) Antibody #9378 |
HeLa |
Untreated |
|
Phospho-PKCθ (Thr538) Antibody #9377 |
Jurkat |
Untreated |
|
Phospho-(Ser/Thr) PKD Substrate Antibody #4381 |
HeLa |
TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PKD/PKCμ (Ser744/748) Antibody #2054 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 30 min) |
|
Phospho-PKD/PKCμ (Ser916) Antibody #2051 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 30 min) |
|
Phospho-PKM2 (Tyr105) Antibody #3827 |
A549 |
Untreated |
|
Phospho-PLCbeta3 (Ser1105) Antibody #2484 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PLCbeta3 (Ser537) Antibody #2481 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-PLCγ1 (Ser1248) Antibody #4510 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008 |
A-431, NIH/3T3 |
Serum-starve overnight, hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-PLCγ1 (Tyr783) Antibody #2821 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-PLCγ2 (Tyr1217) Antibody #3871 |
Ramos, Raji |
Anti-IgM (12 µg/ml for 2 min) |
|
Phospho-PLCγ2 (Tyr759) Antibody #3874 |
Ramos, Raji |
Anti-IgM (12 µg/ml for 2 min) |
|
Phospho-PLD1 (Ser561) Antibody #3834 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PLD1 (Thr147) Antibody #3831 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb #5243 |
HeLa |
Nocadazole (100 ng/ml for 16 hr) |
|
Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb #9062 |
HeLa, HT-29 |
Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off |
|
Phospho-PLK1 (Thr210) Antibody #5472 |
HeLa, HT-29 |
Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml for 24 hr), collect by mitotic shake off |
|
Phospho-PNK1 (Ser114/Thr118) Antibody #3522 |
HeLa |
UV‡ (100 mJ/cm2, 1 hr recovery) |
|
Phospho-PP1α (Thr320) Antibody #2581 |
HeLa |
Nocodazole (400 ng/ml for 16 hr) |
|
PPARγ (C26H12) Rabbit mAb #2435 |
3T3-L1 |
Differentiated into adipocytes for 6 days |
|
Phospho-PRAS40 (Ser183) Antibody #5936 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb #13175 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-PRAS40 (Thr246) Antibody #2640 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb #14041 |
A431 |
hEGF (50 ng/ml for 15 min) |
|
Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611 |
Jurkat |
Untreated or Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Progesterone Receptor (Ser190) Antibody #3171 |
T-47D |
Promegestone (100 nM for 1 hr) |
|
Phospho-Progesterone Receptor (Ser294) Antibody #13736 |
T-47D |
Promegestone (100 nM for 1 hr) |
|
Phospho-Progesterone Receptor (Ser345) Antibody #12783 |
T-47D |
Promegestone (100 nM for 1 hr) |
|
Phospho-PSD93 (Tyr340) Antibody #2930 |
Rat brain |
Ischemia (15 min) follow by reperfusion (4 hr) |
|
Phospho-PSD95 (Ser295) (A8F8Z) Rabbit mAb #45737 |
Mouse brain, rat brain |
Untreated |
Mouse Brain Tissue Control Extracts #55330 |
Phospho-PSD95 (Tyr236/Tyr240) Antibody #3919 |
Rat brain |
Ischemia (15 min) follow by reperfusion (4 hr) |
|
Phospho-PTEN (Ser380) Antibody #9551 |
HeLa |
Untreated |
|
Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549 |
HeLa |
Untreated |
|
Phospho-PTEN (Ser380/Thr382/383) Antibody #9554 |
HeLa |
Untreated |
|
Phospho-PTPα (Tyr789) Antibody #4481 |
293, COS, C6, C2C12 |
Untreated |
|
Phospho-Pyk2 (Tyr402) Antibody #3291 |
HeLa |
Gas6 (400 ng/ml) + H2O2 (4 nM for 10 min) |
|
Phospho-PZR (Tyr241) (D6F9) Rabbit mAb #8131 |
BAEC |
H2O2 (0.3 mM for 30 min) |
|
Phospho-PZR (Tyr263) (D6A5) Rabbit mAb #8088 |
HeLa, 293 |
Untreated |
|
Phospho-PZR (Tyr263) Antibody #5543 |
HeLa, 293 |
Untreated |
|
Phospho-Rac1/cdc42 (Ser71) Antibody #2461 |
A-431 |
Serum-starve 48 hr, hEGF (100 ng/ml for 10 min) |
|
Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb #13404 |
HeLa |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb #6981 |
HeLa |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978 |
HeLa |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Rad18 (Ser403) Antibody #8393 |
HeLa |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-Rad50 (Ser635) Antibody #14223 |
293 |
UV‡ (100 mJ/cm2, 2 hr recovery) |
|
Phospho-RanBP3 (Ser58) Antibody #9380 |
HeLa |
Serum-starve overnight, hEGF (100 ng/ml for 10 min) |
|
Phospho-Raptor (Ser792) Antibody #2083 |
293 |
Oligomycin (0.5 µM for 30 min) |
|
Phospho-Ras-GRF1 (Ser916) Antibody #3321 |
Transfected† |
Transfect with a construct overexpressing mouse Ras-GRF1, Forskolin (30 µM for 5 min) |
|
Phospho-Rb (Ser608) (D10F2) Rabbit mAb #8147 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser608) Antibody #2181 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser780) (C84F6) Rabbit mAb #3590 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser780) (D59B7) Rabbit mAb #8180 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser780) Antibody #9307 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser795) Antibody #9301 |
HT-29 |
Nocadazole (100 ng/ml for 18 hr); treatment induces slight phospho-shift |
Rb Control Proteins #9303 |
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516 |
HeLa, C2C12 |
Untreated |
Rb Control Proteins #9303 |
Phospho-Rb (Ser807/811) Antibody #9308 |
HeLa |
Untreated |
Rb Control Proteins #9303 |
Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb #5500 |
HT-29, HeLa |
Untreated |
|
Phospho-RCC1 (Ser11) Antibody #4210 |
HT-29, HeLa |
Untreated |
|
Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb #5025 |
Jurkat, Raji |
TPA (200 nM for 30 min) |
|
Phospho-RelB (Ser552) Antibody #4999 |
Jurkat, Raji |
TPA (200 nM for 30 min) |
|
Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb #12005 |
Jurkat |
Untreated |
|
Phospho-Ret (Tyr905) Antibody #3221 |
TT |
Untreated |
|
Phospho-Rev-erbα (Ser55/59) Antibody #2129 |
Transfected† |
Transfect with a construct overexpressing human Rev-Erbα |
|
Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-RIP (Ser166) (D1L3S) Rabbit mAb #65746 |
HT-29 |
Z-VAD (20 μM for 30 min) followed by Cycloheximide (10 μg/ml) + hTNF-α (20 ng/ml, overnight) |
|
Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb #14397 |
THP-1 |
TPA (80 nM for 24 hr), LPS (1 µg/ml for 15 min) |
|
ROS1 (D4D6®) Rabbit mAb #3287 |
HCC78 |
Untreated |
|
Phospho-ROS1 (Tyr2274) Antibody #3078 |
HCC78 |
HCC78 cells express a SLC3482-Ros fusion protein |
|
Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb #13499 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-Rpb1 CTD (Ser2/5) Antibody #4735 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-Rpb1 CTD (Ser2/Ser5) (D1G3K) Rabbit mAb #13546 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-Rpb1 CTD (Ser5) (D9N5I) Rabbit mAb #13523 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-Rpb1 CTD (Ser7) (E2B6W) Rabbit mAb #13780 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-Rpb1 CTD (Thr4) Antibody #14934 |
C2C12,
H-4-II-E |
Untreated |
|
Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb #3556 |
HeLa |
TPA (200 nM for 30 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-RSK3 (Thr356/Ser360) Antibody #9348 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 |
MCF7 |
Serum-starve overnight, hIGF-1 (100 ng/ml for 10 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-SAMHD1 (Thr592) Antibody #15038 |
THP-1 |
Untreated |
|
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 |
293 |
UV‡ (40 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 |
293 |
UV‡ (40 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255 |
293 |
UV‡ (40 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251 |
293 |
UV‡ (40 mJ/cm2, 30 min recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-SATB1 (Ser47) Antibody #4028 |
Jurkat |
Untreated |
|
SCD1 (C12H5) Rabbit mAb #2794 |
3T3-L1 |
Differentiated into adipocytes for 8 days |
|
Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb #12316 |
293T, PANC-1, HCT 116 |
Untreated |
|
Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb #4514 |
HeLa |
UV‡ (50 mJ/cm2, 30 min recovery) |
|
Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156 |
HeLa |
UV‡ (50 mJ/cm2, 30 min recovery) |
|
Phospho-SEK1/MKK4 (Ser80) Antibody #9155 |
NIH/3T3 |
hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-SEK1/MKK4 (Thr261) Antibody #9151 |
HeLa |
UV‡ (50 mJ/cm2, 30 min recovery) |
|
Phospho-Semaphorin 4B (Ser825) Antibody #5622 |
MKN-45 |
Untreated |
|
Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit mAb #14390 |
HCT 116 |
Thymidine (2 mM for 18 hr) followed by Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-SF3B1 (Thr313) (D8D8V) Rabbit mAb #25009 |
HeLa, NIH/3T3, COS-7 |
Untreated |
|
Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599 |
SK-MEL-28 |
Untreated |
|
Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb #5642 |
Transfected† |
Transfect with a construct overexpressing human SGK3 |
|
Phospho-SGTA (Ser305) (D23E10) Rabbit mAb #8664 |
HeLa |
Untreated |
|
Phospho-Shc (Tyr239/240) Antibody #2434 |
Hep G2 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
|
Phospho-Shc (Tyr317) Antibody #2431 |
Hep G2 |
Serum-starve overnight, hEGF (100 ng/ml for 5 min) |
|
Phospho-SHIP1 (Tyr1020) Antibody #3941 |
Ramos |
Anti-human IgM (12 µg/ml for 2 min) |
|
Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb #5445 |
K-562 |
Untreated |
|
Phospho-SHIP2 (Tyr1135) Antibody #2007 |
K-562 |
Untreated |
|
Phospho-SHIP2 (Tyr986/987) Antibody #2008 |
HeLa, NIH/3T3, A549 |
Untreated |
|
Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb #8849 |
Jurkat |
Untreated |
|
Phospho-SHP-2 (Tyr542) Antibody #3751 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (PE Conjugate) #13328 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb #5431 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-SHP-2 (Tyr580) Antibody #3703 |
NIH/3T3 |
Serum-starve overnight, hPDGF-BB (100 ng/ml for 5 min) |
|
Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb #14716 |
HeLa, NIH/3T3 |
Serum-starve overnight + Insulin (150nM for 5 min) |
p70 S6 Kinase MCF7 Control Cell Extracts #34499 |
Phospho-SirT1 (Ser27) Antibody #2327 |
293 |
Untreated |
|
Phospho-SirT1 (Ser47) Antibody #2314 |
293 |
Untreated |
|
Phospho-Skp2 (Ser64) Antibody #14865 |
293T, HeLa |
Untreated |
|
Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb #14745 |
Jurkat |
H2O2 (11 mM for 1 min) |
|
Phospho-SLP-76 (Tyr145) Antibody #14770 |
Jurkat |
H2O2 (11 mM for 1 min) |
|
Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb #5753 |
HeLa |
Serum-starve overnight, UV‡ (50 mJ/cm2, 1 hr recovery) |
|
Phospho-Smad1 (Ser206) Antibody #9553 |
HeLa |
Serum-starve overnight, UV‡ (50 mJ/cm2, 1 hr recovery) |
|
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 |
HeLa, HT-1080, C2C12, C6 |
Serum-starve overnight, hBMP-2 (50 ng/ml for 30 min) |
|
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 |
HeLa, HT-1080, C2C12, C6 |
Serum-starve overnight, hBMP-2 (50 ng/ml for 30 min) |
|
Phospho-Smad2 (Ser245/250/255) Antibody #3104 |
HeLa |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 |
HeLa, HT-1080, C2C12, KNRK |
Serum-starve overnight, hTGF-β3 (10 ng/ml for 30 min) |
Smad2/3 Control Cell Extracts #12052 |
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828 |
HT-1080, C2C12, KNRK |
Serum-starve overnight, hTGF-β3 (10 ng/ml for 30 min) |
Smad2/3 Control Cell Extracts #12052 |
Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 |
HT-1080, C2C12, KNRK |
Serum-starve overnight, hTGF-β3 (10 ng/ml for 30 min) |
Smad2/3 Control Cell Extracts #12052 |
Phospho-SMC1 (Ser360) Antibody #4029 |
293 |
UV‡ (100 mJ/cm2, 4 hr recovery) |
|
Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805 |
RAW 264.7, 293 |
UV‡ (100 mJ/cm2, 1 hr recovery) |
SAPK/JNK Control Cell Extracts #9253 |
Phospho-Sox2 (Ser250/Ser251) Antibody #77627 |
NCCIT |
Untreated |
|
Phospho-SQSTM1/p62 (Ser349) Antibody #95697 |
293T |
Sodium arsenite (15 µM, overnight) |
|
Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody #13121 |
ACHN |
Untreated |
|
Phospho-Src (Ser17) (D7F2Q) Rabbit mAb #12432 |
Jurkat |
Untreated |
|
Phospho-Src (Ser17) Antibody #5473 |
MCF7 |
Untreated |
|
Phospho-Src (Tyr527) Antibody #2105 |
COLO-201 |
Untreated |
|
Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 |
COLO-201 |
Untreated |
|
Phospho-Src Family (Tyr416) Antibody #2101 |
COLO-201 |
Untreated |
|
Phospho-SRC-3 (Thr24) Antibody #2979 |
MCF7 |
Untreated |
|
Phospho-SRF (Ser103) Antibody #4261 |
HeLa |
Serum-starve overnight, TPA (200 nM for 15 min) |
MEK1/2 HeLa Control Cell Extracts #9160 |
Phospho-SSH3 (Ser37) Antibody #13873 |
HeLa, ZR-75 |
Calyculin A (100 nM for 30 min) |
|
Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb #8826 |
HeLa |
UV‡ (50 mJ/cm2, 2 hr recovery) |
|
Phospho-Stat1 (Ser727) Antibody #9177 |
HeLa |
UV‡ (50 mJ/cm2, 2 hr recovery) |
|
Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb #88410 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 15 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat2 (Tyr690) Antibody #4441 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 15 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Stat3 (124H6) Mouse mAb #9139 |
HeLa, NIH/3T3, C6 |
Untreated |
Stat1/2/3/5 Control Cell Extracts #9133 |
Acetyl-Stat3 (Lys685) Antibody #2523 |
Transfected† |
Cotransfect with contructs overexpressing human Stat3 and CBP (p300/CREB Binding Protein) |
|
Phospho-Stat3 (Ser727) (6E4) Mouse mAb #9136 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 5–10 min) |
|
Phospho-Stat3 (Ser727) Antibody #9134 |
A-431 |
Serum-starve overnight, hEGF (100 ng/ml for 5–10 min) |
|
Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat3 (Tyr705) Antibody #9131 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134 |
NK-92 |
Cytokine-starve overnight, hIL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth |
|
Phospho-Stat4 (Tyr693) Antibody #5267 |
NK-92 |
Cytokine-starve overnight, hIL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth |
|
Phospho-Stat5 (Tyr694) (14H2) Mouse mAb #9356 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb #4322 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat5 (Tyr694) Antibody #9351 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 5 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364 |
HeLa, Daudi, ACHN |
Serum-starve overnight, hIL-4 (100 ng/ml for 15 min) |
|
Phospho-Stat6 (Tyr641) Antibody #9361 |
HeLa, Daudi, ACHN |
Serum-starve overnight, hIL-4 (100 ng/ml for 15 min) |
|
Phospho-Stathmin (Ser16) Antibody #3353 |
HeLa |
Nocadazole (100 ng/ml for 16 hr) |
|
Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb #4191 |
HT-29 |
Nocodazole (100 ng/mL 24 hours) |
|
Phospho-Stathmin (Ser38) Antibody #3426 |
HT-29 |
Nocodazole (100 ng/ml for 24 hr) |
|
STING (D2P2F) Rabbit mAb #13647 |
HT-29, THP-1 |
Untreated |
|
Phospho-Survivin (Thr34) (D2E11) Rabbit mAb #8888 |
HeLa |
Thymidine (2 mM for 16 hr) followed by Nocadazole (100 mg/ml for 24 hr) |
|
Phospho-Syk (Ser297) Antibody #14140 |
SR |
TPA (200 nM for 15 min) |
|
Phospho-Syk (Tyr323) Antibody #2715 |
Ramos |
Anti-IgM (12 µg/ml for 2 min) |
|
Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710 |
Ramos, RL |
Anti-IgM (12 µg/ml for 2 min) |
|
Phospho-Syk (Tyr525/526) Antibody #2711 |
Ramos, RL |
Anti-IgM (12 µg/ml for 2 min) |
|
Symmetric Di-Methyl Arginine Motif [sdme-RG] Rabbit mAb #13222 |
MCF7 |
Untreated |
|
Symmetric Di-Methyl Arginine Motif [sdme-RG] Rabbit mAb #13522 |
MCF7 |
Untreated |
|
Phospho-Synapsin (Ser9) Antibody #2311 |
Mouse brain, rat brain |
Untreated |
|
Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb #88246 |
Mouse brain, rat brain |
Untreated |
|
Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb #8155 |
293 |
Untreated |
|
Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb #8842 |
HT-29 |
Thymidine (2 mM for 16 hr), add fresh media + Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off |
|
Phospho-TACC3 (Ser558) Antibody #5645 |
HT-29 |
Thymidine (2 mM for 16 hr), add fresh media + Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off |
|
Phospho-TAK1 (Ser412) Antibody #9339 |
HeLa |
hIL-1β (20 ng/ml) + Calyculin A (100 nM for 10 min) |
|
Phospho-TAK1 (Thr184) Antibody #4537 |
HeLa |
hIL-1β (20 ng/ml) + Calyculin A (100 nM for 10 min) |
|
Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508 |
HeLa |
hIL-1β (20 ng/ml) + Calyculin A (100 nM for 10 min) |
|
Phospho-TAK1 (Thr184/187) Antibody #4531 |
HeLa |
hIL-1β (20 ng/ml) + Calyculin A (100 nM for 10 min) |
|
Phospho-TAK1 (Thr187) Antibody #4536 |
HeLa |
hIL-1β (20 ng/ml) + Calyculin A (100 nM for 10 min) |
|
Phospho-Talin (Ser425) (D2P2M) Rabbit mAb #13589 |
293, MCF-7 |
Untreated |
|
Phospho-Talin (Ser425) Antibody #5426 |
293, MCF-7 |
Untreated |
|
Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632 |
Mouse brain, rat brain |
Untreated |
|
Phospho-Tau (Ser416) (D7U2P) Rabbit mAb #15013 |
Mouse brain, rat brain |
Untreated |
|
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb #12885 |
Mouse brain, rat brain |
Untreated |
|
Phospho-TBC1D1 (Ser660) Antibody #6928 |
Transfected† |
Transfect with a construct overexpressing mouse TBC1D1 |
|
Phospho-TBC1D1 (Ser700) Antibody #6929 |
Transfected† |
Transfect with a construct overexpressing mouse TBC1D1 |
|
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483 |
THP-1 |
TPA (80 nM, overnight), LPS (1 µg/ml for 15 min -1 hr) |
|
Phospho-TCTP (Ser46) Antibody #5251 |
HT-29 |
Thymidine (2 mM for 16 hr), add fresh media and Nocadazole (100 ng/ml 24 hr), collect by mitotic shake off |
|
Phospho-Thr-Pro-Arg Motif [pTPR] MultiMab™ Rabbit mAb mix #8142 |
Jurkat |
Calyculin A (10 ng/ml for 20 min) |
Akt control cell extracts #9273 |
Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb #3004 |
HeLa |
Nocadazole (100 ng/ml for 16 hr) |
|
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757 |
HCT 116 |
Thymidine (2 mM for 18 hr) followed by Nocadazole (50 ng/ml for 20 hr) |
|
Phospho-Threonine (42H4) Mouse mAb #9386 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391 |
Jurkat |
Nocadazole (1 µg/ml for 12 hr) |
|
Phospho-Threonine-X-Arginine Antibody #2351 |
Jurkat |
Calyculin A (100 nM for 30 min) |
Akt Control Cell Extracts #9273 |
Phospho-Tie2 (Ser1119) Antibody #4226 |
Transfected† |
Transfect with a construct overexpressing human Tie2 |
|
Phospho-Tie2 (Tyr992) Antibody #4221 |
Transfected† |
Transfect with a construct overexpressing human Tie2 |
|
Phospho-TIF1β (Ser824) Antibody #4127 |
293 |
Hydroxyurea (1.5mM for 18hr) |
|
Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb #5638 |
L-540 |
Untreated - L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein |
|
Phospho-Topoisomerase IIα (Ser1469) (D4F5) Rabbit mAb #13072 |
293T |
Untreated |
|
Phospho-Torc1/Crtc1 (Ser151) Antibody #3359 |
Transfected† |
Transfect with a construct overexpressing human Torc1/Crtc1 |
|
Phospho-Tpl2 (Ser400) Antibody #4491 |
Transfected† |
Transfect with a construct overexpressing rat Tpl2 |
|
Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb #12712 |
M-07e |
Thrombopoietin (100 ng/ml for 10 min) |
|
Phospho-TRAF2 (Ser11) (E2B6L) Rabbit mAb #13908 |
THP-1 |
TPA (80 nM overnight) + LPS (1 µg/ml for 30 min) |
|
Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb #14680 |
HeLa |
Adenosine-2', 3'-dialdehyde (AdOx, 100μM for 24 hr) |
|
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb #4909 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys36) Antibody #9763 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys4) Antibody #9727 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys79) Antibody #4260 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb #13969 |
HeLa, NIH/3T3 |
Untreated |
|
Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb #5737 |
NIH/3T3 |
Untreated |
|
Phospho-TrkA (Tyr490) Antibody #9141 |
PC-12 |
hβ-NGF (100 ng/ml for 2–5 min) |
|
Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 |
PC-12 |
hβ-NGF (100 ng/ml for 2–5 min) |
|
Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 |
PC-12 |
hβ-NGF (100 ng/ml for 2–5 min) |
|
Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb #4168 |
Transfected† |
Transfect with a construct overexpressing human TrkA or TrkB protein |
|
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004 |
Primary cardiac myocytes |
Isoproterenol (1 µM for 5 min) |
|
Phospho-TTF-1 (Ser327) Antibody #13608 |
NCI-H3225, TT, H441 |
Untreated |
|
Phospho-Tuberin/TSC2 (Ser1254) Antibody #3616 |
MCF7, NIH/3T3, C6, Jurkat |
Untreated |
|
Phospho-Tuberin/TSC2 (Ser1387) Antibody #5584 |
SH-SY5Y, 293T, C6 |
Untreated |
|
Phospho-Tuberin/TSC2 (Ser939) Antibody #3615 |
NIH/3T3 |
hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617 |
MCF7 |
hEGF (10 ng/ml for 30 min) |
|
Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 |
MCF7 |
hEGF (10 ng/ml for 30 min) |
|
Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614 |
NIH/3T3 |
hPDGF-BB (50 ng/ml for 30 min) |
|
Phospho-Tyk2 (Tyr1054/1055) Antibody #9321 |
HeLa |
Serum-starve overnight, hIFN-α1 (100 ng/ml for 15 min) |
Stat1/2/3/5 Control Cell Extracts #9133 |
Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (HRP Conjugate) #12695 |
A431 |
Serum-starve, hEGF (100ng/ml for 5 min) |
EGF Control Cell Extracts #5634 |
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954 |
A431 |
Serum-starve, hEGF (100ng/ml for 5 min) |
EGF Control Cell Extracts #5634 |
Phospho-Tyrosine Hydroxylase (Ser31) (D6I9V) Rabbit mAb #13041 |
PC-12 |
Untreated |
|
Phospho-Tyrosine Hydroxylase (Ser31) Antibody #3370 |
PC-12 |
Untreated |
|
Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 |
PC-12 |
Forskolin (30 µM for 5 min) |
|
Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465 |
Jurkat |
Pervanadate (1 mM for 30 min) |
|
Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 |
Jurkat |
Pervanadate (1 mM for 30 min) |
|
Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416 |
Jurkat |
Pervanadate (1 mM for 30 min) |
|
Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb #12753 |
MCF7, MEF |
CCCP (100 µM for 2 hr) |
|
Phospho-ULK1 (Ser467) Antibody #4634 |
Transfected† |
Transfect with a construct overexpressing mouse ULK1 |
|
Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb #5869 |
MCF7 |
Oligomycin (0.5 μM for 30 min) |
|
Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb #14205 |
A172, MCF7 |
Untreated |
|
Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb #14202 |
A172, MCF7 |
Untreated |
|
Phospho-ULK1 (Ser757) Antibody #6888 |
A172, MCF7 |
Untreated |
|
V5-Tag (D3H8Q) Rabbit mAb (#13202) |
Transfected† |
Transfect with a construct overexpressing a V5-tagged protein |
|
Phospho-VASP (Ser157) (D1C8O) Rabbit mAb #84519 |
A-431, NIH/3T3 |
Serum-starve overnight, Forskolin (10 µM for 30 min) |
|
Phospho-VASP (Ser157) Antibody #3111 |
A-431 |
Serum-starve overnight, Forskolin (10 µM for 30 min) |
|
Phospho-VASP (Ser239) Antibody #3114 |
A-431 |
Serum-starve overnight, Forskolin (10 µM for 30 min) |
|
Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817 |
HUVEC |
Serum-starve overnight, hVEGF165 (100 ng/ml for 5 min) |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 |
HUVEC |
Serum-starve overnight, hVEGF165 (100 ng/ml for 5 min) |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb #3770 |
HUVEC |
Serum-starve overnight, hVEGF165 (100 ng/ml for 5 min) |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb #2477 |
Transfected† |
CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991 |
HUVEC |
Serum-starve overnight, hVEGF165 (100 ng/ml for 5 min) |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb #2476 |
Transfected† |
CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr951) Antibody #2471 |
HUVEC |
Serum-starve overnight, hVEGF165 (100 ng/ml for 5 min) |
VEGF Receptor 2 Control Proteins #2904 |
Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474 |
Transfected† |
Transfected with a construct overexpressing human or mouse VEGF Receptor 2 |
VEGF Receptor 2 Control Proteins #2904 |
Vimentin (D21H3) XP® Rabbit mAb #5741 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Phospho-Vimentin (Ser39) Antibody #13614 |
HeLa |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb #7391 |
HeLa |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Vimentin (Ser56) Antibody #3877 |
HeLa |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Vimentin (Ser82) Antibody #3878 |
HeLa |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb #12569 |
HeLa |
Paclitaxel (100 nM for 20 hr) |
|
Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910 |
A-431 |
Serum-starve overnight, hEGF (100 nM for 5 min) |
|
Phospho-WIPI2 (Ser413) Antibody #13571 |
Raji, KARPAS-299 |
Serum-starve overnight, TPA (200 nM for 30 min) |
|
Phospho-WNK1 (Thr60) Antibody #4946 |
HT-29 |
Serum-starve overnight, hIGF-I (100 ng/ml for 30 min) |
|
YAP (D8H1X) XP® Rabbit mAb #14074 |
A-204, C2C12, C6 |
Untreated |
|
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008 |
HeLa, MEF, C6, C2C12 |
Untreated |
|
Phospho-YAP (Ser127) Antibody #4911 |
HeLa, MEF, C6, C2C12 |
Untreated |
|
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619 |
HeLa, MEF, C6, C2C12 |
Untreated |
|
Phospho-YB1 (Ser102) (C34A2) Rabbit mAb #2900 |
MCF7 |
Serum-starve overnight, hIGF-I (100 ng/ml for 30 min) |
|
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717 |
Jurkat |
Anti-CD3 antibody (10 µg/ml for 2 min) |
|
Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701 |
Jurkat |
Anti-CD3 antibody (10 µg/ml for 2 min) |
|
Phospho-Zap-70 (Tyr493) Antibody #2704 |
Jurkat |
Anti-CD3 antibody (10 µg/ml for 2 min) |
|
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467 |
A-431 |
hEGF (100 ng/ml for 30 min) |
|
Phospho-Zyxin (Ser142/143) Antibody #4863 |
A-431 |
hEGF (100 ng/ml for 30 min) |
|
Phospho-α-E-Catenin (Ser652) Antibody #13061 |
MCF-7, NIH/3T3, COS |
Untreated |
|
Phospho-α-E-Catenin (Ser655/Thr658) Antibody #13231 |
HeLa, PANC-1, NIH/3T3 |
Untreated |
|
Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb #12152 |
HeLa |
TSA (400 nM for 16 hr) |
|
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (HRP Conjugate) #14652 |
HeLa |
TSA (400 nM for 16 hr) |
|
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335 |
HeLa |
TSA (400 nM for 16 hr) |
|
Acetyl-α-Tubulin (Lys40) Antibody #3971 |
HeLa |
TSA (400 nM for 16 hr) |
|
β-Actin (13E5) Rabbit mAb #4970 |
HeLa, NIH/3T3, C6 |
Untreated |
|
β-Actin (8H10D10) Mouse mAb #3700 |
HeLa, NIH/3T3, C6 |
Untreated |
|
β-Actin Antibody #4967 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb #2416 |
293 |
Untreated |
|
β-Catenin (D10A8) XP® Rabbit mAb #8480 |
HeLa, NIH/3T3, C6 |
Untreated |
|
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb #9030 |
SW480, SW620 |
Untreated |
|
Acetyl-β-Catenin (Lys49) Antibody #9534 |
SW480, SW620 |
Untreated |
|
Phospho-β-Catenin (Ser33/37) Antibody #2009 |
SW480, SW620 |
Untreated |
|
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 |
SW480, SW620 |
Untreated |
|
Phospho-β-Catenin (Ser45) Antibody #9564 |
SW480, SW620 |
Untreated |
|
Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651 |
SK-N-MC |
Forskolin (50 µM for 30 min) |
|
Phospho-β-Catenin (Ser552) Antibody #9566 |
SK-N-MC |
Forskolin (50 μM for 30 min) |
|
Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176 |
SK-N-MC |
Forskolin (50 μM for 30 min) |
|
Phospho-β-Catenin (Ser675) Antibody #9567 |
SK-N-MC |
Forskolin (50 μM for 30 min) |
|
Phospho-β-Catenin (Thr41/Ser45) Antibody #9565 |
SW480, SW620 |
Untreated |
|
Phospho-μ-Opioid Receptor (Ser375) Antibody #3451 |
Transfected† |
Transfect with a construct overexpressing mouse µ-opioid receptor + DAMGO (1 mM, 20 mins) |
|